PEX19 Antibody (533) - Azide and BSA Free

Western Blot: PEX19 Antibody (533) [NBP2-43757] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 12 % SDS-PAGE PEX19 antibody [533] dilution: 1:500

Immunocytochemistry/Immunofluorescence: PEX19 Antibody (533) [NBP2-43757] - PEX19 antibody (533) detects PEX19 protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PEX19 stained by PEX19 antibody (533) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: PEX19 Antibody (533) [NBP2-43757] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 12 % SDS-PAGE PEX19 antibody [533] dilution: 1:1000.

PEX19 Antibody (533) [NBP2-43757] - PEX19 antibody (533) detects PEX19 protein by flow cytometry analysis.Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: PEX19 antibody (533) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

• Reactivity: Hu, Rt, Mu, Bv
• Applications: WB, Flow, ICC/IF
• Clone: 533
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PEX19 Antibody (533) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PEX19 Antibody (533) - Azide and BSA Free (NBP2-43757) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-PEX19 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.
• Predicted Species: Mouse (91%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PEX19
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for PEX19 Antibody (533) - Azide and BSA Free

• D1S2223Ehousekeeping gene, 33kD
• HK3333 kDa housekeeping protein
• Peroxin-19
• peroxisomal biogenesis factor 19
• Peroxisomal farnesylated proteinFLJ55296
• PMP1
• PMPI
• PXFperoxin-19
• PXMP1

Background

PEX19 is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEX19 Antibody (NBP2-43757)(1)

Publication using NBP2-43757

• Oh J, Kim DK, Ahn SH, Kim HM et Al. A dual role of the conserved PEX19 helix in safeguarding peroxisomal membrane proteins iScience 2024-04-08 [PMID: 38585659]

Bioinformatics

• Gene Symbol: PEX19