PER2 Antibody (JE33-79)

Western Blot: PER2 Antibody (JE33-79) [NBP3-32735] - Western blot analysis of PER2 on K562 cell lysates with Rabbit anti-PER2 antibody (NBP3-32735) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 137 kDa Observed band size: 180 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32735) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PER2 Antibody (JE33-79) [NBP3-32735] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PER2 antibody (NBP3-32735) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32735) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: JE33-79
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PER2 Antibody (JE33-79) Summary

• Description: Novus Biologicals Rabbit PER2 Antibody (JE33-79) (NBP3-32735) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human PER2 aa 50-100. (Uniprot: O15055)
• Localization: Nucleus, Cytoplasm, Perinuclear region; Nucleolus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PER2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:500
• Theoretical MW: 137 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PER2 Antibody (JE33-79)

• Circadian clock protein PERIOD 2
• FASPS
• hPER2
• KIAA0347period 2
• period (Drosophila) homolog 2
• period circadian protein 2
• period circadian protein homolog 2
• period homolog 2 (Drosophila)

Background

PER2 is a member of the Period protein family and a mammalian homolog of the Drosphilia period gene. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Strong PER2 expression in the suprachiasmatic nuclei of the mamalian brain indicates that the PER gene functions in the circadian clock with PER2 being a component of the circadian oscillator. PER2 shares a 40% homology with PER1 including the protein dimerization PAS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PER2