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Human Peptide YY ELISA Kit (Colorimetric)

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ELISA: Human Peptide YY ELISA Kit (Colorimetric) [NBP2-76713] - Standard Curve Reference
ELISA: Human Peptide YY ELISA Kit (Colorimetric) [NBP2-76713] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000pg/mL
Sensitivity
18.75pg/mL

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Human Peptide YY ELISA Kit (Colorimetric) Summary

Standard Curve Range
31.25 - 2000pg/mL
Sensitivity
18.75pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.09
Intra-Assay
CV% < 5.16
Spike Recovery
85-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PYY

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Substrate Reagent
  2. Stop Solution
  3. Reference Standard & Sample Diluent
  4. Certificate of Analysis
  5. Concentrated Wash Buffer (25x)
  6. Concentrated HRP Conjugate (100x)
  7. Concentrated Biotinylated Detection Ab (100x)
  8. Biotinylated Detection Ab Diluent
  9. Reference Standard
  10. Product Description
  11. Plate Sealer
  12. Micro ELISA Plate (Dismountable)
  13. HRP Conjugate Diluent

Alternate Names for Human Peptide YY ELISA Kit (Colorimetric)

  • Peptide tyrosine tyrosine
  • Peptide YY
  • Peptide-YY
  • PYY
  • PYY1
  • PYY-I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
(3 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Cholecystokinin
Antibody
NBP2-62664
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human hypothalamus shows strong positivity in neuronal projections.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human duodenum shows high expression.
Species: Hu
Applications: IHC, IHC-P
Ghrelin Antibody
(883622)
MAB8200
Ghrelin was detected in immersion fixed frozen sections of mouse stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 10&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). Specific staining is shown at both 60x magnification (upper panel) and 20x magnification (lower panel). Staining was localized to cytoplasm and gastric endocrine cells, respectively. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Ghrelin was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 15&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Rat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Publications)
Species: Hu, Mu
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Inorganic
Pyrophosphatase/PPA1
NBP1-31348
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.
Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 &amp; 5 vs. 3 &amp; 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 &amp; 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 &amp; 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 &amp; 6). The difference is minimal in PrP-/- samples (panels 7 &amp; 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
TAP2 Antibody
NBP2-93797
Western Blot: TAP2 Antibody [NBP2-93797] - Analysis of extracts of various cell lines, using TAP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/Immunofluorescence: TAP2 Antibody [NBP2-93797] - Analysis of U2OS cells using TAP2. Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
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Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
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beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog&amp;nbsp;# MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog&amp;nbsp;# FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer&amp;nbsp;I (Catalog&amp;nbsp;# FC005). View our protocol for Staining Intracellular Molecules.
(3 Reviews)
(4 Publications)
Species: Hu, Mu
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RIPK2 Antibody
(6F7)
H00008767-M02
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7. Analysis of RIPK2 expression in NIH/3T3.
Immunohistochemistry-Paraffin: RIPK2 Antibody (6F7) [H00008767-M02] - Analysis of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 1.2 ug/ml.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7 Analysis of RIPK2 expression in HeLa.
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Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue. Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100
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Antibody (7G5)
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Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
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GIP Antibody
(911205) [Unconjugate
MAB8864
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Antibody (JB54-32)
NBP2-75554
Western Blot: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of Proteasome 20S LMP7 on U937 (1) and A431 (2) cell lysate using anti-Proteasome 20S LMP7 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Staining Proteasome 20S LMP7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of paraffin-embedded rat epididymis tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Neurotensin
Antibody
NBP2-33903
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Analysis in human small intestine and skeletal muscle tissues. Corresponding NTS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Species: Hu
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NPY2R Antibody
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Western Blot: NPY2R Antibody [NB110-59978] - analysis of NPY2R in rat hypothalamus lysate using anti-NPY2R antibody. Image from verified customer review.
Immunohistochemistry: NPY2R Antibody [NB110-59978] - Image of NPY Y2 receptor immunoreactivity in mouse dorsal root ganglion of wild type (A) and NPY Y2 receptor Knockout (B) animals. Note that unspecific staining is observed in thick, possibly myelinated fibers.
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Recombinant Human
Gastrin GST (N-Term) ...
H00002520-P01
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POMC Antibody
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Bioinformatics

Gene Symbol PYY