Human Peptide YY ELISA Kit (Colorimetric)

Product Discontinued

Human Peptide YY ELISA Kit (Colorimetric) Summary

• Standard Curve Range: The recommended concentrations are as follows: 100, 50, 25, 12.5, 6.25, 3.125, 1.563, 0 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: Coefficient of variation was <10%.
• Intra-Assay: CV% < 10%.
• Spike Recovery: N/A
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PYY

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human Peptide YY ELISA Kit (Colorimetric)

• Peptide tyrosine tyrosine
• Peptide YY
• Peptide-YY
• PYY
• PYY1
• PYY-I

Background

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Peptide YY ELISA Kit (NBP2-62166)(1)

Publication using NBP2-62166

• Rennekamp R, Brandl B, Giesbertz P et al. Metabolic and satiating effects and consumer acceptance of a fibre-enriched Leberkas meal: a randomized cross-over trial European journal of nutrition 2021-02-08 [PMID: 33555374]

FAQs for Peptide YY ELISA Kit (NBP2-62166). (Showing 1 - 2 of 2 FAQ).

1. I bought the PYY Elisa Kit (NBP2-62166) and wondered about your Standard Curve Range. It´s specified between 31,25 – 2000pg/ml. But the kit contains only a standard with a concentration of 100pg/ml after dilution. How is it possible to get a higher concentration than 100pg/ml with this standard?
   • To fit the value to the range of the standard curve then: If the concentration of the sample exceeds the highest point of the curve or does not lie within the linear range of the curve, then dilute the sample prior to measurement. If a diluted sample is used, remembered to multiply by the dilution factor to obtain the final value.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PYY