Pepsinogen A Antibody (762015) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 762015
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Pepsinogen A Antibody (762015) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A4/PGA4
  Ile16-Ala388
  Accession # P00790
• Specificity: Detects human Pepsinogen A4/PGA4 in direct ELISAs. Human PGA3 and PGA5 reactivity has not been demonstrated but is likely due to their high homology with human PGA4. In direct ELISAs, no cross-reactivity with recombinant human (rh) Pepsinogen C, rhCa
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pepsinogen A Antibody (762015) [Alexa Fluor® 647]

• EC 3.4.23
• EC 3.4.23.1
• FLJ58952
• FLJ77962
• pepsin A
• pepsinogen 4, group I (pepsinogen A)
• Pepsinogen A
• pepsinogen A4
• PGA3
• PGA5

Background

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2‑4 amino acids (aa). Human pepsinogen A isozymogens share approximately 56% amino acid sequence identity with mouse and rat pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.