PEAR1 Antibody

Western blot shows lysates of mouse spleen tissue and mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PEAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PEAR1 at approximately 120-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

bEnd.3 mouse endo­thelioma cell line was stained with Sheep Anti-Mouse PEAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7607, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Jedi is expressed in peripheral glia and endothelium. (A) Cross section of adult (8–12 weeks old) WT sciatic nerve stained for Jedi-1 (magenta) and DAPI (blue). Insets 1 and 2 show Jedi expression in blood vessels. See Supplementary Fig. S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31992767), licensed under a CC-BY license. Not internally tested by R&D Systems.

Jedi is expressed in peripheral glia and endothelium. (A) Cross section of adult (8–12 weeks old) WT sciatic nerve stained for Jedi-1 (magenta) and DAPI (blue). Insets 1 and 2 show Jedi expression in blood vessels. See Supplementary Fig. S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31992767), licensed under a CC-BY license. Not internally tested by R&D Systems.

Jedi is expressed in peripheral glia and endothelium. (A) Cross section of adult (8–12 weeks old) WT sciatic nerve stained for Jedi-1 (magenta) and DAPI (blue). Insets 1 and 2 show Jedi expression in blood vessels. See Supplementary Fig. S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31992767), licensed under a CC-BY license. Not internally tested by R&D Systems.

Jedi is expressed in peripheral glia and endothelium. (A) Cross section of adult (8–12 weeks old) WT sciatic nerve stained for Jedi-1 (magenta) and DAPI (blue). Insets 1 and 2 show Jedi expression in blood vessels. See Supplementary Fig. S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31992767), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PEAR1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse PEAR1
  Leu19-Leu754
  Accession # Q8VIK5
• Specificity: Detects mouse PEAR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human PEAR1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PEAR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEAR1 Antibody

• Jedi
• PEAR1
• platelet endothelial aggregation receptor 1

Background

PEAR1 (Platelet Endothelial Aggregation Receptor 1; Also Jedi/Jagged and Delta protein and MEGF12) is a 140-160 kDa member of the MEGF/Multiple Epidermal Growth Factor domain family of molecules.It has restricted expression, being found on megakaryocytes/platelets, vascular endothelial cells, early hematopoietic stem cells and dorsal root ganglia satellite glial cells. PEAR1 has an extracellular domain (ECD) similar to Jagged1, and soluble PEAR1 is known to be an inhibitor of notch signaling. Thus, it likely participates in blood cell differentiation. It also is reported to bind to apoptotic neurons, facilitating clearance during development. Mature mouse PEAR1 is a 1016 amino acid (aa) type I transmembrane protein. It possesses a 736 aa ECD (aa 19-754) plus a 259 aa cytoplasmic region (aa 776-1034). The ECD contains one EMI (Emilin) domain (aa 25-137), followed by 9 EGF-like repeats (aa 181-691). There are at least three utilized phosphorylation sites in the cytoplasmic tail. Three isoform variants exist. One shows a deletion of aa 375-404, a second contains an 11 aa substitution for aa 737-747, and a third utilizes an alternative start site at Met137. Over aa 19-754, mouse PEAR1 shares 94% and 85% aa sequence identity with rat and human PEAR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEAR1 Antibody (AF7607)(1)

Publication using AF7607

• VE Morrison, MG Houpert, JB Trapani, AA Brockman, PJ Kingsley, KA Katdare, HM Layden, G Nguena-Jon, AJ Trevisan, KA Maguire-Ze, LJ Marnett, GJ Bixa, RA Ihrie, BD Carter Jedi-1/MEGF12-mediated phagocytosis controls the pro-neurogenic properties of microglia in the ventricular-subventricular zone bioRxiv : the preprint server for biology, 2023-03-06;0(0):. 2023-03-06 [PMID: 36945622] (IHC, Mouse)

Bioinformatics

• Gene Symbol: PEAR1
• Uniprot:
  • Q8VIK5()