PEAR1 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

PEAR1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse PEAR1
  Leu19-Leu754
  Accession # Q8VIK5
• Specificity: Detects mouse PEAR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human PEAR1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEAR1 Antibody [Alexa Fluor® 750]

• Jedi
• PEAR1
• platelet endothelial aggregation receptor 1

Background

PEAR1 (Platelet Endothelial Aggregation Receptor 1; Also Jedi/Jagged and Delta protein and MEGF12) is a 140-160 kDa member of the MEGF/Multiple Epidermal Growth Factor domain family of molecules.It has restricted expression, being found on megakaryocytes/platelets, vascular endothelial cells, early hematopoietic stem cells and dorsal root ganglia satellite glial cells. PEAR1 has an extracellular domain (ECD) similar to Jagged1, and soluble PEAR1 is known to be an inhibitor of notch signaling. Thus, it likely participates in blood cell differentiation. It also is reported to bind to apoptotic neurons, facilitating clearance during development. Mature mouse PEAR1 is a 1016 amino acid (aa) type I transmembrane protein. It possesses a 736 aa ECD (aa 19-754) plus a 259 aa cytoplasmic region (aa 776-1034). The ECD contains one EMI (Emilin) domain (aa 25-137), followed by 9 EGF-like repeats (aa 181-691). There are at least three utilized phosphorylation sites in the cytoplasmic tail. Three isoform variants exist. One shows a deletion of aa 375-404, a second contains an 11 aa substitution for aa 737-747, and a third utilizes an alternative start site at Met137. Over aa 19-754, mouse PEAR1 shares 94% and 85% aa sequence identity with rat and human PEAR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.