PDIA6 Antibody (JF0974)

Western Blot: PDIA6 Antibody (JF0974) [NBP2-67031] - Analysis of PDIA6 on different lysates using anti-PDIA6 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: K562

Immunocytochemistry/Immunofluorescence: PDIA6 Antibody (JF0974) [NBP2-67031] - Staining PDIA6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: PDIA6 Antibody (JF0974) [NBP2-67031] - Analysis of Hela cells with PDIA6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PDIA6 Antibody (JF0974) [NBP2-67031] - Staining PDIA6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PDIA6 Antibody (JF0974) [NBP2-67031] - Staining PDIA6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

PDIA6 Antibody (JF0974) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within PDIA6 aa 1-108 / 440. (SwissProt: Q15084 Human; SwissProt: Q922R8 Mouse; SwissProt: Q63081 Rat)
• Localization: Endoplasmic reticulum lumen, Cell membrane, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDIA6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Proximity Ligation Assay
  • Western Blot 1:1000-1:2000
• Application Notes: Use in Proximity Ligation Assay reported in scientific literature (PMID:34929376)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDIA6 Antibody (JF0974)

• EC 5.3.4.1
• Endoplasmic reticulum protein 5
• ERp5
• ERP5ER protein 5
• P5TXNDC7
• protein disulfide isomerase family A, member 6
• Protein disulfide isomerase P5
• protein disulfide isomerase-associated 6
• protein disulfide isomerase-related protein
• protein disulfide-isomerase A6
• thioredoxin domain containing 7 (protein disulfide isomerase)
• Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerase (PDIA6) ia an enzyme belonging to the protein disulfide isomerase family. Structurally, it contains 2 thioredoxin domains and its function is to catalyze the rearrangement of -S-S- bonds in proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDIA6 Antibody (NBP2-67031)(1)

Publication using NBP2-67031

• Bouchalova P, Sommerova L, Potesil D et al. Characterization of the AGR2 interactome uncovers new players of Protein Disulfide Isomerase network in cancer cells Molecular & cellular proteomics : MCP 2021-12-17 [PMID: 34929376] (PLA, Human)

Bioinformatics

• Gene Symbol: PDIA6