PDIA6 Antibody (3B4) - Azide and BSA Free

Western Blot: PDIA6 Antibody (3B4) [H00010130-M04] - Analysis of PDIA6 expression in transfected 293T cell line by PDIA6 monoclonal antibody (M04), clone 3B4. Lane 1: PDIA6 transfected lysatE (48.1 KDa). Lane 2: Non-transfected lysate.

ELISA: PDIA6 Antibody (3B4) [H00010130-M04] - Detection limit for recombinant GST tagged PDIA6 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDIA6 Antibody (3B4) - Azide and BSA Free Summary

• Immunogen: PDIA6 (AAH01312, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDRNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEGDIDLSDVELDDLGKDEL
• Specificity: Reacts with protein disulfide isomerase family A, member 6.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDIA6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDIA6 Antibody (3B4) - Azide and BSA Free

• EC 5.3.4.1
• Endoplasmic reticulum protein 5
• ERp5
• ERP5ER protein 5
• P5TXNDC7
• protein disulfide isomerase family A, member 6
• Protein disulfide isomerase P5
• protein disulfide isomerase-associated 6
• protein disulfide isomerase-related protein
• protein disulfide-isomerase A6
• thioredoxin domain containing 7 (protein disulfide isomerase)
• Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDIA6 Antibody (H00010130-M04)(2)

Publications using H00010130-M04

• Sasha S, Ekram G, Erik R et al. Tumor-associated autoantibodies from mouse breast cancer models are found in serum of breast cancer patients. NPJ Breast Cancer. 2021-05-11 [PMID: 33976232]
• Lee S, Min Kim S, Dotimas J et al. Thioredoxin-interacting protein regulates protein disulfide isomerases and endoplasmic reticulum stress. EMBO Mol Med. 2014-05-19 [PMID: 24843047]

Bioinformatics

• Gene Symbol: PDIA6
• Entrez:
  • 10130(Human)
• Uniprot:
  • AAH01312(Human)