PDE10A Antibody (OTI1C9) - Azide and BSA Free

Western Blot: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Ovary tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded bladder tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded colon tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded endometrium tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry: PDE10A Antibody (OTI1C9) - Azide and BSA Free [NBP2-73273] - Staining of paraffin-embedded prostate tissue using anti-PDE10A mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PDE10A Antibody (OTI1C9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PDE10A Antibody (OTI1C9) - Azide and BSA Free (NBP1-47909) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human PDE10A expression vector
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE10A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 88.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PDE10A Antibody (OTI1C9) - Azide and BSA Free

• cAMP and cAMP-inhibited cGMP 3'-5'-cyclic phosphodiesterase 10A
• dJ416F21.1 (phosphodiesterase 10A)
• EC 3.1.4
• EC 3.1.4.17
• EC 3.1.4.35
• FLJ11894
• FLJ25677
• HSPDE10A
• PDE10A
• phosphodiesterase 10A
• phosphodiesterase 10A1 (PDE10A1)

Background

One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE10A