PDE10A Antibody (OTI1C9)

Western Blot: PDE10A Antibody (1C9) [NBP1-47909] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] Staining of paraffin-embedded prostate tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded bladder tissue using anti-PDE10Amouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded colon tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded endometrium tissue using anti-PDE10A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Staining of paraffin-embedded Ovary tissue using anti-PDE10A mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PDE10A Antibody (OTI1C9) Summary

• Description: Novus Biologicals Mouse PDE10A Antibody (OTI1C9) (NBP1-47909) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human PDE10A expression vector
• Specificity: This antibody is specific for Homo sapiens phosphodiesterase 10A (PDE10A), transcript variant 2.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE10A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 88.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE10A Antibody (OTI1C9)

• cAMP and cAMP-inhibited cGMP 3'-5'-cyclic phosphodiesterase 10A
• dJ416F21.1 (phosphodiesterase 10A)
• EC 3.1.4
• EC 3.1.4.17
• EC 3.1.4.35
• FLJ11894
• FLJ25677
• HSPDE10A
• PDE10A
• phosphodiesterase 10A
• phosphodiesterase 10A1 (PDE10A1)

Background

Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3'cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. There are 11 subtypes of PDEs, named PDE1-11; PDE4, 7 and 8 selectively degrade cAMP, PDE5, 6 and 9 selectively degrade cGMP and PDE1, 2, 3, 10 and 11 degrade both cyclic nucleotides. PDEs are expressed ubiquitously,with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility,platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE10A
• Entrez:
  • 10846(Human)