PDCD7 Antibody (46C713.2.1)

Western Blot: PDCD7 Antibody (46C713.2.1) [NB100-56540] - analysis PDCD-7 using PDCD-7 monoclonal antibody. Mouse NIH3T3 cells were probed with PDCD-7 antibody at 2 ug/ml.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 46C713.2.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDCD7 Antibody (46C713.2.1) Summary

• Immunogen: The antibody was raised against a synthetic peptide of mouse PDCD-7 protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 28134623)
  • Western Blot 1-2 ug/ml
• Control:

  NIH 3T3 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for PDCD7 Antibody (46C713.2.1)

• apoptosis-related protein ES18
• ES18programmed cell death protein 7
• FLJ54213
• HES18
• MGC22015
• programmed cell death 7
• U11/U12 snRNP 59K

Background

PDCD7 (programmed cell death protein 7) was identified by screening EST database. The expression pattern implies that the role of PDCD7/ES18 seems to be associated with the immune system, and it may be selectively involved in specific apoptotic pathway and able to effect cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDCD7 Antibody (NB100-56540)(1)

Publication using NB100-56540

• Berlato C, Khan MN, Schioppa T et al. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer. J. Clin. Invest. 2017-03-01 [PMID: 28134623] (ICC/IF, Mouse)

FAQs for PDCD7 Antibody (NB100-56540). (Showing 1 - 1 of 1 FAQ).

1. Can you tell me the peptide sequence for the PDCD7 Antibody (46C713.2.1) immunogen? I am considering of ordering this to test its cross-reactivity against zebrafish pdcd7, but want to know if the region is conserved.
   • The exact sequence of the immunogen for our product NB100-56540 is proprietary. I can tell you that the immunogen is two separate peptide sequences from the mouse PDCD7 protein that share 31.2% and 58% homology to the ZebraFish PDCD7 protein. Based on these alignments, I would not recommend this product for ZebraFish detection. We did have two items to this target that had higher homologies to the ZebraFish protein that you might be interested in: NBP2-13743 (68%) and NBP1-59082 (60%). Since we have not validated ZebraFish detection for any of our PDCD7 antibodies, if you do decide to try any of our products in your samples, you would qualify for our Innovator's Reward Program. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply purchase the product as normal and submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Gene Symbol: PDCD7
• Entrez:
  • 50996(Mouse)
• Uniprot:
  • Q8N8D1(Human)