PDCD4 Antibody (k4C1) - BSA Free

Western Blot: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04329 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate.

Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP1-04329 (1:100). The secondary antibody (green) used was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow Cytometry: PDCD4 Antibody (k4C1) - BSA Free [NBP1-04329] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP1-04329 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: k4C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PDCD4 Antibody (k4C1) - BSA Free Summary

• Immunogen: Recombinant human PDCD4 (1-469aa) purified from E. coli (NP_055271).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PDCD4 Antibody (k4C1) - BSA Free

• H731
• H731nuclear antigen H731
• MGC33046
• MGC33047
• Neoplastic transformation inhibitor protein
• Nuclear antigen H731-like
• PDCD4
• programmed cell death 4 (neoplastic transformation inhibitor)
• programmed cell death protein 4
• Protein 197/15a

Background

Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDCD4 Antibody (NBP1-04329)(1)

Publication using NBP1-04329

• Abraham D, Jackson N, Gundara JS et al. MicroRNA profiling of sporadic and hereditary medullary thyroid cancer identifies predictors of nodal metastasis, prognosis, and potential therapeutic targets Clin Cancer Res 2011-07-15 [PMID: 21622722] (WB, Human)

Bioinformatics

• Gene Symbol: PDCD4
• Entrez:
  • 27250(Human)
• Uniprot:
  • Q53EL6(Human)