PDCD4 Antibody (4C2) - BSA Free

Western Blot: PDCD4 Antibody (4C2) [NBP3-18224] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-18224 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: K562 cell lysate. Lane 2: HeLa cell lysate. Lane 3: 293T cell lysate

Immunocytochemistry/Immunofluorescence: PDCD4 Antibody (4C2) [NBP3-18224] - ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with NBP3-18224 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow Cytometry: PDCD4 Antibody (4C2) [NBP3-18224] - Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with NBP3-18224 at 2-5ug for 1x10^6cells (red). A Goat anti-mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 4C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PDCD4 Antibody (4C2) - BSA Free Summary

• Description: Novus Biologicals Mouse PDCD4 Antibody (4C2) - BSA Free (NBP3-18224) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PDCD4 (1-469aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate-Buffered Saline (pH 7.4), 10% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDCD4 Antibody (4C2) - BSA Free

• H731
• H731nuclear antigen H731
• MGC33046
• MGC33047
• Neoplastic transformation inhibitor protein
• Nuclear antigen H731-like
• PDCD4
• programmed cell death 4 (neoplastic transformation inhibitor)
• programmed cell death protein 4
• Protein 197/15a

Background

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD4