PCK1 Antibody

Images

 
Western Blot: PCK1 Antibody [NBP1-54825] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.
Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-54825] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: PCK1 Antibody [NBP1-54825] - Human Fetal Brain Lysate, concentration 1.0ug/ml.
Western Blot: PCK1 Antibody [NBP1-54825] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.
Western Blot: PCK1 Antibody [NBP1-54825] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.
Western Blot: PCK1 Antibody [NBP1-54825] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: PCK1 Antibody [NBP1-54825] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Western Blot: PCK1 Antibody [NBP1-54825] - MCF7, Antibody Dilution: 1.0 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PCK1 Antibody Summary

Immunogen
Synthetic peptides corresponding to PCK1 (phosphoenolpyruvate carboxykinase 1 (soluble)) The peptide sequence was selected from the middle region of PCK1. Peptide sequence NGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 4.0-8.0 ug/ml
  • Immunohistochemistry-Paraffin 4.0-8.0 ug/ml
Application Notes
This is a rabbit polyclonal antibody against PCK1 and was validated on Western Blot and immunohistochemistry-P
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-54825.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for PCK1 Antibody

  • EC 4.1.1.32
  • MGC22652
  • PCK1
  • PEPCK1
  • PEPCKC
  • PEPCK-C
  • PEPCK-CPEP carboxykinase
  • phosphoenolpyruvate carboxykinase 1 (soluble)
  • phosphoenolpyruvate carboxykinase, cytosolic [GTP]
  • phosphoenolpyruvate carboxykinase, cytosolic
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCK1 Antibody (NBP1-54825)(1)

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PCK1
Uniprot