PCK1 Antibody

Images

 
Western Blot: PCK1 Antibody [NB100-60556] - Western blot analysis of PCK1 using NB100-60556 at 0.5ug/ml in human Kidney lysate (35ug protein in RIPA buffer).
Western Blot: PCK1 Antibody [NB100-60556] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PCK1 Antibody Summary

Immunogen
Peptide with sequence C-EKEVEDIEKYLEDQ corresponding to internal region (near C-Terminus) according to NP_002582.2.
Epitope
EKEVEDIEKYLEDQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 70 kDa band observed in human kidney lysates (calculated MW of 69.2 kDa band according to NP_002582.2).
Positive Control
PCK1 Lysate (NBL1-14179)
Publications
Read Publication using NB100-60556.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PCK1 Antibody

  • EC 4.1.1.32
  • MGC22652
  • PCK1
  • PEPCK1
  • PEPCKC
  • PEPCK-C
  • PEPCK-CPEP carboxykinase
  • phosphoenolpyruvate carboxykinase 1 (soluble)
  • phosphoenolpyruvate carboxykinase, cytosolic [GTP]
  • phosphoenolpyruvate carboxykinase, cytosolic
  • Phosphoenolpyruvate carboxylase
  • phosphopyruvate carboxylase

Background

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCK1 Antibody (NB100-60556)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PCK1 Antibody (NB100-60556) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCK1
Entrez
Uniprot