PCK1 Antibody

Product Discontinued

PCK1 Antibody Summary

• Immunogen: Peptide with sequence C-EKEVEDIEKYLEDQ corresponding to internal region (near C-Terminus) according to NP_002582.2.
• Epitope: EKEVEDIEKYLEDQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human kidney lysates (calculated MW of 69.2 kDa band according to NP_002582.2).
• Control:

  PCK1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PCK1 Antibody

• EC 4.1.1.32
• MGC22652
• PCK1
• PEPCK1
• PEPCKC
• PEPCK-C
• PEPCK-CPEP carboxykinase
• phosphoenolpyruvate carboxykinase 1 (soluble)
• phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• phosphoenolpyruvate carboxykinase, cytosolic
• Phosphoenolpyruvate carboxylase
• phosphopyruvate carboxylase

Background

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCK1 Antibody (NB100-60556)(1)

Publication using NB100-60556

• Willer CJ, Bonnycastle LL, Conneely KN et al. Screening of 134 single nucleotide polymorphisms (SNPs) previously associated with type 2 diabetes replicates association with 12 SNPs in nine genes. Diabetes 2007-01-01 [PMID: 17192490]

Bioinformatics

• Gene Symbol: PCK1
• Entrez:
  • 5105(Human)