PCDHA6 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC206238 and based on accession number NM_018909. The protein contains a C-terminal DDK tag.
Homo sapiens protocadherin alpha 6 (PCDHA6), transcript variant 1, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for PCDHA6
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for PCDHA6 Overexpression Lysate (Native)
- KIAA0345-like 8
- protocadherin alpha 6
- protocadherin alpha-6
This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq]. Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt, Po, Bt, Bv, Ca, Eq, Mk, Pm
Applications: ICC/IF, IHC-P, ICC
Species: Hu, Mu, Rt
Applications: WB, ELISA
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Applications: IHC, IHC-P
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Applications: WB, Flow, ICC/IF, IHC-P
Applications: WB, ELISA, ICC/IF, IHC-P, IP
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Publications for PCDHA6 Lysate (NBL1-14153) (0)
There are no publications for PCDHA6 Lysate (NBL1-14153).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for PCDHA6 Lysate (NBL1-14153) (0)
There are no reviews for PCDHA6 Lysate (NBL1-14153).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for PCDHA6 Lysate (NBL1-14153) (0)
Additional PCDHA6 Products
Bioinformatics Tool for PCDHA6 Lysate (NBL1-14153)
Discover related pathways, diseases and genes to PCDHA6 Lysate (NBL1-14153). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Diseases for PCDHA6 Lysate (NBL1-14153)
Discover more about diseases related to PCDHA6 Lysate (NBL1-14153).
Pathways for PCDHA6 Lysate (NBL1-14153)
View related products by pathway.
Blogs on PCDHA6