PCBP1 Antibody

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Western Blot: PCBP1 Antibody [NBP1-52113] - Western blot analysis of PCBP1 (aa223-234) in PBMC lysate (35ug protein in RIPA buffer) using NBP1-52113 at 0.1ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

PCBP1 Antibody Summary

Immunogen
Peptide with sequence C-SIQGQHTISPLD corresponding to internal region according to NP_006187.2.
Epitope
C-SIQGQHTISPLD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 38 kDa band observed in human peripheral blood nucleocytes lysates (calculated MW of 37.5 kDa band according to NP_006187.2). The 38 kDa band was also observed in rat spleen while a 37 kDa band was observed in rat thymus.
Publications
Read Publication using NBP1-52113.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PCBP1 Antibody

  • alpha-CP1
  • Heterogeneous nuclear ribonucleoprotein E1
  • heterogenous nuclear ribonucleoprotein E1
  • heterogenous nuclear ribonucleoprotein X
  • hnRNP E1
  • hnRNP-E1
  • hnRNP-X
  • HNRPE1
  • HNRPX
  • nucleic acid binding protein sub 2.3
  • Nucleic acid-binding protein SUB2.3
  • poly(rC) binding protein 1
  • poly(rC)-binding protein 1

Background

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCBP1 Antibody (NBP1-52113)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PCBP1
Entrez
Uniprot