Pax5/BSAP Antibody (1H9) [PE]

Images

 
Flow Cytometry: Pax5/BSAP Antibody (1H9) [PE] [NBP2-00257] - Surface staining of C57BL/6 splenocytes with Anti-Mouse CD23 PE-Cy7 and Anti-Mouse CD21/CD35 FITC followed by intracellular staining using the Foxp3 Staining ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
1H9
Clonality
Monoclonal
Host
Rat
Conjugate
PE

Pax5/BSAP Antibody (1H9) [PE] Summary

Immunogen
The immunogen for this antibody is Pax5, BSAP (1H9).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PAX5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This 1H9 antibody has been tested by western blot analysis. This can be used at less than or equal to 5ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This 1H9 antibody has also been reported for use in immunoprecipitation and immunohistology staining of frozen tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Pax5/BSAP Antibody (1H9) [PE]

  • B cell specific activator protein
  • B-cell-specific transcription factor
  • BSAP
  • BSAPB-cell lineage specific activator
  • paired box 5
  • paired box gene 5 (B-cell lineage specific activator protein)
  • paired box gene 5 (B-cell lineage specific activator)
  • paired box homeotic gene 5
  • paired box protein Pax-5
  • Pax5
  • transcription factor PAX 5

Background

The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VHDJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAX5
Entrez