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PATL1 Antibody

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Western Blot: PATL1 Antibody [NBP2-83366] - Host: Rabbit. Target Name: PATL1. Sample Tissue: Human Liver Tumor. Antibody Dilution: 1.0ug/ml
Western Blot: PATL1 Antibody [NBP2-83366] - Host: Rabbit. Target Name: PATL1. Sample Tissue: Human Liver Tumor. Antibody Dilution: 1.0ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PATL1 Antibody Summary

Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human PATL1. Peptide sequence: ATPPPQQHPPGPGPHLQNLRSQAPMFRPDTTHLHPQHRRLLHQRQQQNRS The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Gene
PATL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PATL1 Antibody

  • FLJ36874
  • hPat1b
  • MGC125671
  • OK/KNS-cl.5
  • Pat1b
  • PAT1-like protein 1
  • protein associated with topoisomerase II homolog 1 (yeast)
  • protein PAT1 homolog 1
  • protein PAT1 homolog b

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DDX6 Antibody
NB200-191
Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. Image submitted by a verified customer review.
(1 Review)
(38 Publications)
Species: Ha, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
SLC36A1 Antibody
NBP2-80524
SLC36A1 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
APPBP2 Antibody -
BSA Free
NBP2-81781
Western Blot: APPBP2 Antibody [NBP2-81781] - Analysis of APPBP2 in human brain tissue lysate with APPBP2 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunohistochemistry-Paraffin: APPBP2 Antibody [NBP2-81781] - APPBP2 in human brain tissue with APPBP2 antibody at 5 ug/mL.
Immunocytochemistry/Immunofluorescence: APPBP2 Antibody [NBP2-81781] - Immunofluorescence of APPBP2 in human brain tissue with APPBP2 antibody at 20 ug/mL.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
LSM1 Antibody
(OTI5C6)
NBP2-00918
Flow Cytometry: LSM1 Antibody (5C6) [NBP2-00918] - Analysis of Hela cells, using anti-LSM1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: LSM1 Antibody (5C6) [NBP2-00918] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LSM1 antibody, and then analyzed by flow cytometry.
Western Blot: LSM1 Antibody (5C6) [NBP2-00918] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
YSK4 Antibody (2A4)
H00080122-M01
Immunocytochemistry/Immunofluorescence: YSK4 Antibody (2A4) [H00080122-M01] - Immunofluorescence of monoclonal antibody to YSK4 on HeLa cell. [antibody concentration 40 ug/ml]
Immunocytochemistry/Immunofluorescence: YSK4 Antibody (2A4) [H00080122-M01] - Analysis of monoclonal antibody to YSK4 on HeLa cell . Antibody concentration 40 ug/ml.
ELISA: YSK4 Antibody (2A4) [H00080122-M01] - Detection limit for recombinant GST tagged YSK4 is 1 ng/ml as a capture antibody.
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF
IFIT2 Antibody
NBP1-31164
Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP1-31164] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.
(2 Reviews)
(6 Publications)
Species: Hu
Applications: ICC/IF, WB
Xrn1 Antibody
NB500-191
Western Blot: Xrn1 Antibody [NB500-191] - Detection of human and mouse XRN1 by western blot. Samples:Whole cell lysate (15 ug) from GaMG, HEK293T, RKO, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN1 antibody NB500-191 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
(7 Publications)
Species: Hu, Mu
Applications: IP, WB
Splicing factor,
arginine/serine-rich ...
NBP1-87381
Immunohistochemistry-Paraffin: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Analysis in human endometrium and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.
Western Blot: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Analysis in control (vector only transfected HEK293T lysate) and SRSF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ets-1 Antibody
[Unconjugated]
AF7284
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human thymus tissue, mouse thymus tissue, and rat CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Ets-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ets-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ets‑1 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human/Mouse/Rat Ets‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Species: Hu, Mu, Rt
Applications: IHC, WB
Dcp1a Antibody
(3G4)
H00055802-M06
Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - The MIWI2/MAEL granule is a modified P-body. Localization of P-bodies in gonocytes. Cross section seminiferous tubule from wild-type testis (E18.5) were probed with antibodies against DCP1a. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1000764), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Localization of P-bodies in gonocytes. Co-localization of MAEL and P-body component DCP1a in piP-bodies. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1000764), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Anti-Dcp1alpha antibody recognizes its antigen. SCG neuron shown overexpressing GFP-Dcp1alpha. Note that all GFP-Dcp1alpha granules are recognized by anti-Dcp1alpha antibody. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/cddis2013297), licensed under a CC-BY licence.
(1 Review)
(42 Publications)
Species: Hu, I, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IP, PLA, WB
NONO Antibody
NB100-1556
Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.
(2 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IHC, IHC-P, IP, WB
FKBP51/FKBP5
Antibody [Unconjugate
AF4094
Western blot shows lysates of DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP51 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FKBP51 at approximately 61 kDa (as indicated) using 3 µg/mL of Goat Anti-Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
FKBP51 was detected in immersion fixed paraffin-embedded sections of human prostate using 15 µg/mL Human/Mouse/Rat FKBP51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4094) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
EDC4 Antibody
NBP2-13944
Western Blot: EDC4 Antibody [NBP2-13944] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-13944] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-13944] - Staining of human Colon shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
DCP2 Antibody
NBP2-16109
Western Blot: DCP2 Antibody [NBP2-16109] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-16109] - Zebrafish blastula cells expressing GFP-Rbpms2 fusions. Wild-type GFP-Rbpms2b localizes near the nucleus (top row), is apparently associated with the centrosome/spindle in some cells. (Top row) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2. (Second row) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (Third rod) GFP-Rbpms2bae32 and (Final row) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pgen.1007489) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: DCP2 Antibody [NBP2-16109] - Mouse testis. DCP2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
(2 Publications)
Species: Hu, Mu, Ze
Applications: ICC/IF, IHC, IHC-P, ISH, WB
PDCD6 Antibody
[Unconjugated]
AF6015
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PDCD6 Polyclonal Antibody (Catalog # AF6015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Species: Hu, Mu, Rt
Applications: WB
eIF4ENIF1 Antibody
H00056478-B01P
Immunocytochemistry/Immunofluorescence: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of purified antibody to EIF4ENIF1 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of EIF4ENIF1 expression in Hela S3 NE.
Western Blot: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of EIF4ENIF1 expression in transfected 293T cell line by EIF4ENIF1 polyclonal antibody. Lane 1: EIF4ENIF1 transfected lysate(108.35 KDa). Lane 2: Non-transfected lysate.
(3 Publications)
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC/IF, IP, WB
RNPC3 Antibody
NBP2-93867
Western Blot: RNPC3 Antibody [NBP2-93867] - Analysis of extracts of various cell lines, using RNPC3 . Exposure time: 60s.
Species: Hu, Mu, Rt
Applications: WB
NRAS Antibody
(6S6F3)
NBP3-16473
Immunocytochemistry/Immunofluorescence: NRAS Antibody (6S6F3) [NBP3-16473] - Immunofluorescence analysis of U-2 OS cells using NRAS Rabbit mAb (NBP3-16473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: NRAS Antibody (6S6F3) [NBP3-16473] - Analysis of extracts of various cell lines, using NRAS Rabbit mAb (NBP3-16473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: NRAS Antibody (6S6F3) [NBP3-16473] - Analysis of extracts of Rat lung, using NRAS Rabbit mAb (NBP3-16473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Species: Hu, Mu, Rt
Applications: ICC/IF, WB

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Bioinformatics

Gene Symbol PATL1