Patched 1/PTCH Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Patched 1/PTCH Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant mouse Patched 1/PTCH
  Glu108-Asp422
  Accession # Q61115
• Specificity: Detects mouse Patched 1/PTCH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Patched 1 is observed, and less than 5% cross-reactivity with recombinant mouse Patched-2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Patched 1/PTCH Antibody [Alexa Fluor® 488]

• BCNS
• BCNSFLJ42602
• FLJ26746
• HPE7
• mes
• NBCCS
• patched (Drosophila) homolog
• Patched 1
• patched homolog (Drosophila)
• patched homolog 1 (Drosophila)
• patched homolog 1
• protein patched homolog 1
• PTC
• ptc1
• PTCH protein +12b
• PTCH protein +4'
• PTCH protein -10
• PTCH
• PTCH1
• PTCH11

Background

Patched (Ptch1) is a 170‑175 kDa protein that functions in Hedgehog signaling. It is found on multiple cell types and forms a constitutive, inactivating complex with Smoothened. Upon Hedgehog binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Ptch1 has been reported in mice. However, two or more splice forms may exist, with variation occurring over the first 150 aa of the N-terminus. Mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Patched 1/PTCH Antibody (AF4105G). (Showing 1 - 1 of 1 FAQ).

1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
   • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.