PAPSS1 Antibody (1F4) - Azide and BSA Free

Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4. Lane 1: PAPSS1 transfected lysatE (68.5 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of monoclonal antibody to PAPSS1 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi or non-transfected control. Blot probed with H00009061-M05. GAPDH (36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 1F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PAPSS1 Antibody (1F4) - Azide and BSA Free Summary

• Immunogen: PAPSS1 (NP_005434, 144 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAE
• Specificity: PAPSS1 - 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAPSS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PAPSS1 Antibody (1F4) - Azide and BSA Free

• 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• ATPSK1SK 1
• bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• EC 2.7.1.25
• PAPS synthase 1
• PAPSS 1
• PAPSS3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1
• SK1
• Sulfurylase kinase 1

Background

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAPSS1
• Entrez:
  • 9061(Human)
• OMIM:
  • 603262(Human)
• Uniprot:
  • NP_005434(Human)