Pappalysin-2/PAPP-A2 Antibody (242011)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 242011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pappalysin-2/PAPP-A2 Antibody (242011) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
  Ser234-Cys1396
  Accession # Q9BXP8
• Specificity: Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human PAPP-A is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAPPA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pappalysin-2/PAPP-A2 Antibody (242011)

• EC 3.4.24.-
• PAPPA2
• PAPP-A2
• PAPP-A2pappalysin-2
• pappalysin 2
• Pappalysin2
• Pappalysin-2
• PAPPE
• PAPP-E
• PLAC3
• placenta-specific 3
• pregnancy-associated plasma preproprotein-A2
• Pregnancy-associated plasma protein A2
• Pregnancy-associated plasma protein E1

Background

Pappalysins belong to a fifth family of metzincin proteases that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2.

1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pappalysin-2/PAPP-A2 Antibody (MAB1668)(1)

Publication using MAB1668

• Nishizawa H, Pryor-Koishi K, Suzuki M Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod., 2008-09-18;14(10):595-602. 2008-09-18 [PMID: 18805800] (ELISA Development, Western Blot, Human)

Bioinformatics

• Gene Symbol: PAPPA2
• Uniprot:
  • Q9BXP8()