Pannexin-1 Antibody (720505) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 720505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Pannexin-1 Antibody (720505) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Pannexin-1
  Pro298-Asp376
  Accession # Q96RD7
• Specificity: Detects human Pannexin-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Pannexin-2 or
  -3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pannexin-1 Antibody (720505) [Alexa Fluor® 350]

• Innexin
• MGC21309
• MRS1
• MRS1innexin
• pannexin 1
• Pannexin1
• Pannexin-1
• PANX1
• PX1
• UNQ2529

Background

Pannexin-1 (PANX1) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed on epithelial cells, neuronal and glial cells, cardiac myocytes, T cells, and erythrocytes. Pannexin-1 forms large pore hexameric channels or heteromeric channels with Pannexin-2 and enables the efflux of ATP and UTP. Its conductance is increased in response to hypoxia, isotonic stress, NMDA R activation, and Caspase-3 and -7 activation during apopotosis. Extracellular release of ATP through Pannexin-1 activates P2X7 receptors which in turn induces the closure of Pannexin-1 channels. Pannexin-1 cooperates with P2X7 for inflammasome activation, Caspase-1 activation, and the release of mature IL-1 beta and also regulates T cell activation at the immunological synapse. Within aa 298-376 (in the C-terminal cytoplasmic domain), human Pannexin-1 shares 96% and 98% aa sequence identity with mouse and rat Pannexin-1, respectively. Alternate splicing generates an additional isoform that lacks the C-terminal four amino acids in the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.