Pannexin-3 Antibody (788905)

Western blot shows lysates of SW1353 human chondrosarcoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pannexin-3 Monoclonal Antibody (Catalog # MAB8169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pannexin-3 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clone: 788905
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pannexin-3 Antibody (788905) Summary

• Immunogen: E. coli-derived recombinant human Pannexin-3
  Tyr289-Thr384
  Accession # Q96QZ0
• Specificity: Detects human Pannexin-3 in ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PANX3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pannexin-3 Antibody (788905)

• gap junction protein pannexin 3
• pannexin 3
• Pannexin3
• Pannexin-3
• PANX3
• PX3

Background

Pannexin-3 (PANX3) is an approximately 45 kDa member of the Pannexin family of four-transmembrane channel proteins with a conserved pattern of cysteines. It is expressed in skin, cochlea, and developing hard tissues including cartilage and bone. In osteoblasts, it forms Ca2+ channels in the endoplasmic reticulum and promotes osteogenic differentiation. It also forms gap junction channels that pass Ca2+ between cells, and ATP-releasing hemichannels in the plasma membrane. Within aa 289-384 (in the C-terminal cytoplasmic domain), human Pannexin-3 shares 95% and 99% aa sequence identity with mouse and rat Pannexin-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pannexin-3 Antibody (MAB8169)(1)

Publication using MAB8169

• J Boucher, C Simonneau, G Denet, J Clarhaut, AC Balandre, M Mesnil, L Cronier, A Monvoisin Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis Int J Mol Sci, 2018-05-24;19(6):. 2018-05-24 [PMID: 29882918] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PANX3
• Uniprot:
  • Q96QZ0()