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Pancreatic Polypeptide/PP Peptide

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Summary
Reactivity HuSpecies Glossary
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Pancreatic Polypeptide/PP Peptide Summary

Description
A peptide to PPY.
Protein/Peptide Type
Peptide
Gene
PPY

Applications/Dilutions

Dilutions
  • Antibody Competition
Application Notes
This peptide is useful as a blocking peptide for NB100-1793.

For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Preservative
No Preservative

Alternate Names for Pancreatic Polypeptide/PP Peptide

  • INN
  • Obinepitide
  • pancreatic polypeptide (ppy)
  • pancreatic polypeptide PNPPP
  • pancreatic polypeptide Y
  • Pancreatic Polypeptide
  • pancreatic prohormone
  • PNP
  • PP
  • PPY

Background

Pancreatic Polypeptide is a pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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(182410) [Unconjugate ...
MAB1417
Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(16 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
Somatostatin
Antibody (7G5) - BSA
NBP2-37447
Immunofluorescence: Somatostatin Antibody (7G5) [NBP2-37447] - Paraffin-embedded human adult pancreas tissue. pH 9 antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
ELISA: Somatostatin Antibody (7G5) [NBP2-37447] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
(1 Review)
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
Neuropeptide Y
Antibody
NBP1-46535
Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY licence.
(15 Publications)
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
Peptide YY
Antibody (1) - BSA Fr
NBP1-05167
Immunohistochemistry-Paraffin: Peptide YY Antibody (1) - BSA Free [NBP1-05167] - Paraffin-embedded human small intestine tissue .
Immunohistochemistry-Paraffin: Peptide YY Antibody (1) - BSA Free [NBP1-05167] - Paraffin-embedded human small intestine tissue .
Sandwich ELISA Capture: Peptide YY Antibody (1) - BSA Free [NBP1-05167] - Calibration curve for PYY using NBP1-05167 as the capture antibody and NBP1-05167B as the biotinylated detection antibody.
Species: Hu
Applications: ELISA, IHC, IHC-P, ELISA(Cap), S-ELISA
Cholecystokinin
Antibody
NBP2-62664
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human skeletal muscle shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
Gastrin Antibody
NBP3-12223
Immunohistochemistry-Paraffin: Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.
Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.
Species: Hu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
VIP Antibody
(OTI1E8)
NBP2-46349
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu
Applications: IHC, WB
GIP Antibody
(911205) [Unconjugate
MAB8864
GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
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RIPK2 Antibody
(6F7)
H00008767-M02
ELISA: RIPK2 Antibody (6F7) [H00008767-M02] - Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemistry-Paraffin: RIPK2 Antibody (6F7) [H00008767-M02] - Analysis of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 1.2 ug/ml.
Western Blot: RIPK2 Antibody (6F7) [H00008767-M02] - RIPK2 monoclonal antibody (M02), clone 6F7 Analysis of RIPK2 expression in HeLa.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Secretin Antibody
NBP2-48988
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining in human duodenum and liver tissues using anti-SCT antibody. Corresponding SCT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Secretin Antibody [NBP2-48988] - Staining of human liver shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
Neurotensin
Antibody
NBP2-33903
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Species: Hu
Applications: IHC, IHC-P
Motilin Antibody
NBP2-93868
Immunohistochemistry-Paraffin: Motilin Antibody [NBP2-93868] - Paraffin-embedded human stomach using Motilin .
Immunohistochemistry-Paraffin: Motilin Antibody [NBP2-93868] - Paraffin-embedded mouse brain using Motilin .
Immunohistochemistry-Paraffin: Motilin Antibody [NBP2-93868] - Paraffin-embedded mouse heart using Motilin .
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Gastrin-releasing
Peptide/Bombesin/Neur ...
NBP3-03282
Immunocytochemistry/Immunofluorescence: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Immunofluorescence analysis of HeLa cells using Gastrin-releasing Peptide/Bombesin/Neuromedin C antibody (NBP3-03282) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Rat brain using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Gastrin-releasing Peptide/Bombesin/Neuromedin C Antibody [NBP3-03282] - Mouse heart using Gastrin-releasing Peptide/Bombesin/Neuromedin C Rabbit pAb (NBP3-03282) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Adenosine
Deaminase/ADA Antibod
NBP1-87404
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
POMC Antibody
NB100-1533
Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
TFF2 Antibody
(366508) [Unconjugate
MAB4077
TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(1 Publication)
Species: Hu
Applications: IHC, WB
Substance P
Antibody (266815)
MAB4375
Substance P was detected in immersion fixed paraffin-embedded sections of human spinal cord using 8 µg/mL Human/Mouse/Rat Substance P Monoclonal Antibody (Catalog # MAB4375) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC

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Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Pancreatic Polypeptide/PP Antibody
NB100-1793

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Bioinformatics

Gene Symbol PPY