Pancreatic Amylase Beta Overexpression Lysate (Native)

Images

 
Western Blot: Pancreatic Amylase Beta Overexpression Lysate (Native) [NBL1-07510] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Pancreatic Amylase Beta Overexpression Lysate (Native).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Pancreatic Amylase Beta Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203812 and based on accession number NM_020978. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Pancreatic Amylase Beta Overexpression Lysate (Native), mRNA.
Gene
AMY2B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Pancreatic Amylase Beta

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Pancreatic Amylase Beta Overexpression Lysate (Native)

  • alpha-amylase 2B
  • AMY2
  • AMY2B amylase, alpha 2B (pancreatic)

Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AMY2B