PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free

Western Blot: PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free [NBP2-73234] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP5.

Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free [NBP2-73234] - Analysis of Jurkat cells, using anti-MPP5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free [NBP2-73234] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MPP5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free [NBP2-73234] - Analysis of Hela cells, using anti-MPP5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI2D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human MPP5(NP_071919) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PALS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 77.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PALS1/MPP5 Antibody (OTI2D3) - Azide and BSA Free

• MAGUK p55 subfamily member 5
• membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• MPP5
• PALS1
• PALS1FLJ12615
• Stardust

Background

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001). MPP5 is a member of the p55-like MAGUK subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PALS1