Palladin Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, PC-3 human prostate cancer cell line, and Caki-2 human clear cell carcinoma epithelial cell line. PVDF Membrane was probed with 0.5 µg/mL of Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). Specific bands were detected for Palladin at approximately 95, 115, and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Palladin was detected in immersion fixed paraffin-embedded sections of Human Prostate using Sheep Anti-Human Palladin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5950) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Palladin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Palladin
  Met1154-Gly1304
  Accession # Q8WX93
• Specificity: Detects human Palladin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PALLD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Palladin Antibody [Unconjugated]

• CGI151
• CGI-151
• FLJ22190
• FLJ38193
• KIAA0992FLJ39139
• MYN
• myoneurin
• Palladin
• palladin, cytoskeletal associated protein
• PALLD
• PNCA1
• Sarcoma antigen NY-SAR-77
• SIH002
• SIH002FLJ61376

Background

Palladin (a cytoarchitectural protein named after the 16th century Venetian architect Palladio) is a cytoplasmic member of the myotilin/palladin family of proteins. Palladin is widely expressed and interacts with actin-associated proteins, thus promoting actin bundle stability. Human Palladin is 1383 amino acids (aa) in length and contains five I‑type Ig-like domains (aa 271-360; 440-539; 1001-1085; 1135-1226; 1233-1324), a Pro-rich region (aa 634-864) and nine potential Ser/Thr phosphorylation sites. Multiple isoform variants exist. Predominant are a 140 kDa and 92-95 kDa short form that show alternative start sites at Met383 and Met712, respectively. The 140 kDa isoform also shows a deletion of aa 656-879 to generate an approximately 115 kDa isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PALLD
• Uniprot:
  • Q8WX93()