PACT Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

PACT Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human PACT
  Ser2-Lys313
  Accession # O75569
• Specificity: Detects human PACT in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PACT Antibody [Alexa Fluor® 405]

• DYT16
• DYT16interferon-inducible double stranded RNA-dependent activator
• HSD14
• PACT
• PACTPKR-associating protein X
• PKR-associated protein X
• PKR-Associating Protein X
• PRKRA
• protein kinase, interferon-inducible double stranded RNA dependent activator
• RAX
• RAXHSD14

Background

PACT (PKR Activator; also HSD14, PRKRA and RAX [in mouse]) is a 34-37 kDa member of the PRKRA family of molecules. It is a ubiquitously expressed, cytoplasmic, 313 amino acid (aa) protein that contains three discrete double-stranded (ds) RNA binding motifs (aa 31-101; 126-194; 240-308). It exists as a monomer, homodimer, and heterodimer with at least two partners, TRBP and Dicer. Two isoform variants are known to exist, including one that utilizes an alternative start site at Met26, and another that shows a 10 aa substitution for aa 1-21. PACT is perhaps best known for its relationship with PKR, a 52-58 kDa Ser/Thr kinase that downmodulates protein synthesis and initiates apoptosis. PKR is constitutively silent, and activated via two principal ways; the first is through binding to (viral) dsRNA, and the second is through binding to an activator, PACT. PACT is kept in check upstream through dimerization with at 48-49 kDa regulatory protein termed TRBP. Following growth factor withdrawl or exposure to bacterial products such as LPS, PACT is phosphorylated at Ser287, and this inactive PACT:TRBP heterodimer dissociates, freeing up PACT to bind to PKR. PACT's interaction with PKR induces an autophosphorylation event on PKR. This allows for an activated PKR interaction with eIF2a, causing its phosphorylation and a subsequent reduction of protein synthesis. PACT may also act independently of PKR, and serve as a component of RISC, a complex that downregulates mRNA translation. Within this complex, PACT likely associates with Dicer in concert with Argonaute. Full-length PACT shares 98% aa sequence identity with the mouse ortholog to human PACT termed RAX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.