PACT Antibodies
We offer PACT Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each PACT Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PACT Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the PACT Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PACT polyclonal antibodies and browse our PACT monoclonal antibody catalog.
Alternate Names for PACT Antibodies
anti-PACT antibody, anti-PRKRA antibody, anti-DYT16interferon-inducible double stranded RNA-dependent activator antibody, anti-PACTPKR-associating protein X antibody, anti-PKR-associated protein X antibody, anti-protein kinase, interferon-inducible double stranded RNA dependent activator antibody, anti-RAXHSD14 antibody
9 Results for "pact" in Primary Antibodies
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7. Analysis of PRKRA expression in human liver.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0013.jpg "Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7. Analysis of PRKRA expression in human liver.")
![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0009.jpg)
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7 Analysis of PRKRA expression in HeLa.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0012.jpg)
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.Lane 1: PRKRA transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0007.jpg)
![Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunoprecipitation-H00008575-M01-img0010.jpg)
![ELISA: PACT Antibody (1B9-1A7) [H00008575-M01] - Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-ELISA-H00008575-M01-img0015.jpg)
![Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32705, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-298202414333692.jpg "Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32705, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32705) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32705) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29820241435228.jpg)
![Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-14) [NBP3-32705] - Immunocytochemistry analysis of 293T cells labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29202410484893.jpg)
![Flow Cytometry: PACT Antibody (PSH02-14) [NBP3-32705] - Flow cytometric analysis of 293T cells labeling PACT. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32705, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29202416372161.jpg)
![Western Blot: PACT Antibody (PSH02-14) [NBP3-32705] - Western blot analysis of PACT on different lysates with Rabbit anti-PACT antibody (NBP3-32705) at 1/2,000 dilution. Lane 1: K-562cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-3920248453520.jpg)
![Western Blot: PACT Antibody (PSH02-15) [NBP3-32706] - Western blot analysis of PACT on different lysates with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: K-562 cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Hela cell lysate Lane 7: HL-60 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse testis tissue lysate Lane 11: Mouse liver tissue lysate Lane 12: Rat testis tissue lysate Lane 13: Rat brain tissue lysate Cell lysates: 20ug/lane, tissue lysates: 40ug/lane Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32706) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-278202415212435.jpg "Western Blot: PACT Antibody (PSH02-15) [NBP3-32706] - Western blot analysis of PACT on different lysates with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: K-562 cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Hela cell lysate Lane 7: HL-60 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse testis tissue lysate Lane 11: Mouse liver tissue lysate Lane 12: Rat testis tissue lysate Lane 13: Rat brain tissue lysate Cell lysates: 20ug/lane, tissue lysates: 40ug/lane Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32706) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunofluorescence analysis of paraffin-embedded rat cerebral cortex tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32706, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-298202414304121.jpg)
![Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32706) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29820241420964.jpg)
![Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-15) [NBP3-32706] - Immunocytochemistry analysis of 293T cells labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PACT antibody (NBP3-32706) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29202410334052.jpg)
![Flow Cytometry: PACT Antibody (PSH02-15) [NBP3-32706] - Flow cytometric analysis of 293T cells labeling PACT. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32706, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29202416453248.jpg)
![Western Blot: PACT Antibody [NBP2-55124] - Analysis using Anti-PRKRA antibody NBP2-55124 (A) shows similar pattern to independent antibody NBP2-55123 (B).](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55124-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PACT Antibody [NBP2-55124] - Analysis using Anti-PRKRA antibody NBP2-55124 (A) shows similar pattern to independent antibody NBP2-55123 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PACT Antibody [NBP2-55124] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55124-img0001.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55124] - Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunohistochemistry-Paraffin-NBP2-55124-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PACT Antibody [NBP2-55124] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55124-img0005.jpg)
![Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PACT-Antibody-2W2E10-Western-Blot-NBP3-16701-img0004.jpg "Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded human brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025209816.jpg)
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded mouse brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025209843.jpg)
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded rat brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025208496.jpg)
![Western Blot: PACT Antibody [NBP2-55123] - Analysis using Anti-PRKRA antibody NBP2-55123 (A) shows similar pattern to independent antibody NBP2-55124 (B).](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55123-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PACT Antibody [NBP2-55123] - Analysis using Anti-PRKRA antibody NBP2-55123 (A) shows similar pattern to independent antibody NBP2-55124 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PACT Antibody [NBP2-55123] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55123-img0001.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55123] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunohistochemistry-Paraffin-NBP2-55123-img0002.jpg)
![Western Blot: PACT Antibody (S04-8F7) [NBP3-15072] - Western blot detection of PACT in C6, 3T3, Hela cell lysates using PACT Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/PACT-Antibody-S04-8F7-Western-Blot-NBP3-15072-img0001.jpg "Western Blot: PACT Antibody (S04-8F7) [NBP3-15072] - Western blot detection of PACT in C6, 3T3, Hela cell lysates using PACT Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
![Western Blot: PACT Antibody [NBP2-19674] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-19674-img0001.jpg "Western Blot: PACT Antibody [NBP2-19674] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.")