| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55124-25ul | |||
HAF017). A specific band was detected for PACT at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PACT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1980) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PACT at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1980-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55123-25ul | |||
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7. Analysis of PRKRA expression in human liver.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0013.jpg "Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7. Analysis of PRKRA expression in human liver.")
![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0009.jpg)
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7 Analysis of PRKRA expression in HeLa.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0012.jpg)
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.Lane 1: PRKRA transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0007.jpg)
![Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-Immunoprecipitation-H00008575-M01-img0010.jpg)
![ELISA: PACT Antibody (1B9-1A7) [H00008575-M01] - Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PACT-Antibody-1B9-1A7-ELISA-H00008575-M01-img0015.jpg)
![Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32705, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-298202414333692.jpg "Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32705, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32705) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32705) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29820241435228.jpg)
![Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-14) [NBP3-32705] - Immunocytochemistry analysis of 293T cells labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29202410484893.jpg)
![Flow Cytometry: PACT Antibody (PSH02-14) [NBP3-32705] - Flow cytometric analysis of 293T cells labeling PACT.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32705, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32705_rabbit-pact-mab-psh02-14-29202416372161.jpg)
![Western blot analysis on different lysates with NBP3-32705 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32705 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32705_-western-blot-639204595248916664.jpg)
![Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunofluorescence analysis of paraffin-embedded rat cerebral cortex tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32706, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-298202414304121.jpg "Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunofluorescence analysis of paraffin-embedded rat cerebral cortex tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32706, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: PACT Antibody (PSH02-15) [NBP3-32706] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32706) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32706) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29820241420964.jpg)
![Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-15) [NBP3-32706] - Immunocytochemistry analysis of 293T cells labeling PACT with Rabbit anti-PACT antibody (NBP3-32706) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PACT antibody (NBP3-32706) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29202410334052.jpg)
![Flow Cytometry: PACT Antibody (PSH02-15) [NBP3-32706] - Flow cytometric analysis of 293T cells labeling PACT.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32706, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32706_rabbit-pact-mab-psh02-15-29202416453248.jpg)
![Western blot analysis on different lysates with NBP3-32706 at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: K-562 cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Hela cell lysate Lane 7: HL-60 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse testis tissue lysate Lane 11: Mouse liver tissue lysate Lane 12: Rat testis tissue lysate Lane 13: Rat brain tissue lysate Cell lysates: 20ug/lane, tissue lysates: 40ug/lane Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32706 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32706_-western-blot-639204595249011244.jpg)
![Western Blot: PACT Antibody [NBP2-55124] - Analysis using Anti-PRKRA antibody NBP2-55124 (A) shows similar pattern to independent antibody NBP2-55123 (B).](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55124-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PACT Antibody [NBP2-55124] - Analysis using Anti-PRKRA antibody NBP2-55124 (A) shows similar pattern to independent antibody NBP2-55123 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PACT Antibody [NBP2-55124] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55124-img0001.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55124] - Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunohistochemistry-Paraffin-NBP2-55124-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PACT Antibody [NBP2-55124] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55124-img0005.jpg)
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PACT-Antibody-2W2E10-Western-Blot-NBP3-16701-img0004.jpg "Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded human brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025209816.jpg)
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded mouse brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025209843.jpg)
![Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded rat brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16701_rabbit-pact-mab-2w2e10-822025208496.jpg)
![Western Blot: PACT Antibody [NBP2-55123] - Analysis using Anti-PRKRA antibody NBP2-55123 (A) shows similar pattern to independent antibody NBP2-55124 (B).](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-55123-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PACT Antibody [NBP2-55123] - Analysis using Anti-PRKRA antibody NBP2-55123 (A) shows similar pattern to independent antibody NBP2-55124 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PACT Antibody [NBP2-55123] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55123-img0001.jpg)
![Immunohistochemistry-Paraffin: PACT Antibody [NBP2-55123] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PACT-Antibody-Immunohistochemistry-Paraffin-NBP2-55123-img0002.jpg)
![Western Blot: PACT Antibody [NBP2-19674] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PACT-Antibody-Western-Blot-NBP2-19674-img0001.jpg "Western Blot: PACT Antibody [NBP2-19674] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PACT Antibody (S04-8F7) [NBP3-15072] - Western blot detection of PACT in C6, 3T3, Hela cell lysates using PACT Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/PACT-Antibody-S04-8F7-Western-Blot-NBP3-15072-img0001.jpg "Western Blot: PACT Antibody (S04-8F7) [NBP3-15072] - Western blot detection of PACT in C6, 3T3, Hela cell lysates using PACT Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
