PACSIN3 Antibody

Western Blot: PACSIN3 Antibody [NB100-862] - Staining (1 ug/ml) of H460 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PACSIN3 Antibody Summary

• Description: Novus Biologicals Goat PACSIN3 Antibody (NB100-862) is a polyclonal antibody validated for use in WB and ELISA. Anti-PACSIN3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence APEEDAGGEALGGS-C corresponding to N-Terminus according to NP_057307.2.
• Localization: Cytoplasmic. Detected at the inner aspect of the plasma membrane in cells or myotubes.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PACSIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot
• Application Notes: WB: Staining of human skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
• Control:

  PACSIN3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PACSIN3 Antibody

• Endophilin I
• FLJ17861
• protein kinase C and casein kinase substrate in neurons 3
• protein kinase C and casein kinase substrate in neurons protein 3
• SDPIII
• SH3 domain-containing protein 6511
• syndapin III

Background

PACSIN 3 differs from the other family members in having a short proline-rich region and lacking asparagine-proline-phenylalanine motifs. In contrast to the neurospecific PACSIN 1 and the ubiquitously expressed PACSIN 2, PACSIN 3 is mainly detected in lung and muscle tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PACSIN3 Antibody (NB100-862)(1)

Publication using NB100-862

• Cuajungco MP, Grimm C, Oshima K et al. PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4. J Biol Chem 2006-07-07 [PMID: 16627472]

Bioinformatics

• Gene Symbol: PACSIN3
• Entrez:
  • 29763(Human)
  • 80708(Mouse)
  • 311187(Rat)
• Uniprot:
  • NP_057307.(Human)