PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) - Azide and BSA Free

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) [H00005721-M01] - Analysis of PSME2 expression in transfected 293T cell line by PSME2 monoclonal antibody (M01), clone 3F11-1A3.Lane 1: PSME2 transfected lysate(27.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) [H00005721-M01] - Analysis of monoclonal antibody to PSME2 on HeLa cell . Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3F11-1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSME2 (AAH19885, 1 a.a. ~ 239 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVPKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY
• Specificity: PSME2 - proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSME2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PA28 Activator beta Subunit/PSME2 Antibody (3F11-1A3) - Azide and BSA Free

• 11S regulator complex beta subunit
• 11S regulator complex subunit beta
• Activator of multicatalytic protease subunit 2
• MCP Activator
• MCP activator, 31-kD subunit
• PA28 Activator beta Subunit
• PA28b
• PA28betacell migration-inducing protein 22
• proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Proteasome activator 28 subunit beta
• proteasome activator 28-beta
• proteasome activator complex subunit 2
• proteasome activator hPA28 subunit beta
• PSME2
• REGbeta
• REG-beta

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSME2
• Entrez:
  • 5721(Human)
• OMIM:
  • 602161(Human)
• Uniprot:
  • AAH19885(Human)