Recombinant Human PA28 Activator alpha Subunit/PSME1 Protein

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SDS-Page: PA28 Activator alpha Subunit/PSME1 Recombinant Protein [NBP1-49437] - 15% SDS-PAGE LoadingR: reducing sample bufferNR: non-reducing sample buffer

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human PA28 Activator alpha Subunit/PSME1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-249 of Human PA28 Activator alpha Subunit/PSME1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PSME1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PA28 Activator alpha Subunit/PSME1 Protein

  • 11S regulator complex alpha subunit
  • 11S Regulator Complex Subunit alpha
  • 29-kD MCP activator subunit
  • IFI5111
  • IFI5111MGC8628
  • IGUP I-5111
  • Interferon gamma up-regulated I-5111 protein
  • interferon-gamma IEF SSP 5111
  • interferon-gamma-inducible protein 5111
  • PA28 Activator alpha Subunit
  • PA28A
  • PA28alphaActivator of multicatalytic protease subunit 1
  • proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
  • Proteasome activator 28 subunit alpha
  • proteasome activator complex subunit 1
  • proteasome activator subunit-1
  • PSME1
  • REG alpha
  • REGalpha
  • REG-alpha

Background

PSME1 is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring. Recombinant human PSME1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PSME1
Entrez