p73 Antibody

Western Blot: p73 Antibody [NB100-56435] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate using this antibody.

Western Blot: p73 Antibody [NB100-56435] - Western blot analysis of HeLa cell lysate (nuclear fraction) using p73 antibody at 3 ug/ml.

Simple Western: p73 Antibody [NB100-56435] - Simple Western lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

p73 Antibody Summary

• Immunogen: A synthetic peptide CTHSPYAQPSS corresponding to amino acids 95-104 of human p73 was used as immunogen.
• Specificity: This peptide sequence is 100% conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Researchers are encourage to use the the immunogen sequence information and available Bioinformatics data bases to obtain additional information regarding the amino acid sizes associated with various p73 isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP73
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:12.5
  • Western Blot 1-3 ug/ml
• Application Notes: Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described (Sayan et al, 2008). The ability of a given antibody to recognize a p73 fragment will depend on whether or not the antibody epitope is contained in the fragment. As p73 research continues to evolve, researchers are encouraged to refer to the published literature using the p73 antibodies to gain additional information.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  p73 Overexpression Lysate

Reactivity Notes

Predicted to react with Chicken, Chimpanzee, Canine, Mouse, New World Monkey and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p73 Antibody

• p53-related protein
• P73p53-like transcription factor
• TA p73
• TAp73
• tumor protein p73

Background

p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP73
• Entrez:
  • 7161(Human)
• Uniprot:
  • NP_005418(Human)