p70 S6 Kinase/S6K Antibody

Images

 
Western Blot: p70 S6 Kinase/S6K Antibody [NB100-1104] - Staining (0.2ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using ...read more
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody [NB100-1104] - (3.8ug/ml) staining of paraffin embedded Human Kidney Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

p70 S6 Kinase/S6K Antibody Summary

Immunogen
Peptide with sequence C-MISKRPEHLRMNL corresponding to C-Terminus according to NP_003152.1.
Epitope
C-MISKRPEHLRMNL
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPS6KB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 60 kDa band observed in human placenta lysates (calculated MW of 59.1 kDa band according to NP_003152.1). IHC-P: Human kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus.
Positive Control
p70 S6 Kinase/S6K Lysate (NBL1-15574)
Skeletal Muscle Lysate (NB820-59253)
Publications
Read Publication using NB100-1104.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

p70 S6 kinase (p70S6K, p85S6K) or ribosomal protein S6 kinase has 2 isoforms both of which are monomers. The first isoform, p70, has a predicted molecular mass of 56 kDa and an apparent molecular mass (SDS-PAGE) of 70 kDa. The second isoform, p85 which contains 23 extra residues at the N-terminus, is generated by alternative splicing and has a predicted molecular mass of 59 kDa and an apparent molecular mass of 85 kDa. Serine phosphorylation of the S6 kinase is required for its activation, and this is accompanied by mobility shift in the migration of this 65 kDa kinase on SDA polyacrylamide gels to 70 kDa. Phosphorylation of the S6 protein has been linked with increased rates of initiation of protein synthesis with certain mRNA transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p70 S6 Kinase/S6K Antibody (NB100-1104)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on p70 S6 Kinase/S6K.

S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.

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Bioinformatics

Gene Symbol RPS6KB1
Entrez
Uniprot