p70 S6 Kinase/S6K Antibody (215247) [Phycoerythrin]

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat p70 S6 Kinase PE-conjugated Monoclonal Antibody (Catalog # IC8962P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu, Rt
• Applications: Flow
• Clone: 215247
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

p70 S6 Kinase/S6K Antibody (215247) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human p70 S6 Kinase
  Accession # M60725
• Specificity: Detects human, mouse, and rat p70 S6 Kinase and p85 S6 Kinase in Western blots. Reactivity with beta isoforms of p70 S6 Kinase is unknown.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p70 S6 Kinase/S6K Antibody (215247) [Phycoerythrin]

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20‑fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1) (1-3). Over amino acids (aa) 1-502, human and mouse share 99% aa sequence identity.

1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
3. Fenton, T.R. and  I.T. Gout (2011) Int. J. Biochem. Cell Biol. 43:47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p70 S6 Kinase/S6K Antibody (IC8962P)(1)

Publication using IC8962P

• AT Kwok, NS Mohamed, JF Plate, RR Yammani, S Rosas, TA Bateman, E Livingston, JE Moore, BA Kerr, J Lee, CM Furdui, L Tan, ML Bouxsein, VL Ferguson, LS Stodieck, DC Zawieja, MD Delp, XW Mao, JS Willey Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents Scientific Reports, 2021-05-18;11(1):10469. 2021-05-18 [PMID: 34006989] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: RPS6KB1
• Uniprot:
  • M60725()