P2X5/P2RX5 Overexpression Lysate (Native)

Images

 
Western Blot: P2X5 Overexpression Lysate (Adult Normal) [NBL1-14031] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2X5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

P2X5/P2RX5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207923 and based on accession number NM_002561. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 5 (P2RX5), transcript variant 1, mRNA.
Gene
P2RX5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for P2X5/P2RX5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for P2X5/P2RX5 Overexpression Lysate (Native)

  • ATP receptor subunit
  • ATP receptor
  • ionotropic ATP receptor P2X5
  • LRH-1
  • MGC47755
  • P2RX5
  • P2X purinoceptor 5
  • P2X5
  • P2X5lymphoid-restricted histocompatibility antigen-1
  • P2X5R
  • purinergic receptor P2X ligand gated ion channel 5
  • purinergic receptor P2X, ligand-gated ion channel, 5
  • Purinergic receptor

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript, and it encodes the longest isoform (A).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol P2RX5
Entrez