P2X5/P2RX5 Antibody

Western Blot: P2X5/P2RX5 Antibody [NBP1-82441] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-408

Immunocytochemistry/Immunofluorescence: P2X5/P2RX5 Antibody [NBP1-82441] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunohistochemistry-Paraffin: P2X5/P2RX5 Antibody [NBP1-82441] - Staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells.

Immunohistochemistry-Paraffin: P2X5/P2RX5 Antibody [NBP1-82441] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

Product Discontinued

P2X5/P2RX5 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TLMKAYGIRFDVMVNGKGAFFCDLVLIYLIKKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P2RX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for P2X5/P2RX5 Antibody

• ATP receptor subunit
• ATP receptor
• ionotropic ATP receptor P2X5
• LRH-1
• MGC47755
• P2RX5
• P2X purinoceptor 5
• P2X5
• P2X5lymphoid-restricted histocompatibility antigen-1
• P2X5R
• purinergic receptor P2X ligand gated ion channel 5
• purinergic receptor P2X, ligand-gated ion channel, 5
• Purinergic receptor

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Three transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P2RX5