| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP |
| Clone | SX53G8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 488 |
| Immunogen | Purified GST-p27 fusion protein of human origin |
| Localization | Nuclear |
| Specificity | This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDKN1B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for p27/Kip1 Antibody (NBP2-34718AF488)Discover more about diseases related to p27/Kip1 Antibody (NBP2-34718AF488).
| Pathways for p27/Kip1 Antibody (NBP2-34718AF488)View related products by pathway.
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![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/fullsize2/p21-CIP1-CDKN1A-Antibody-(WA-1-(HJ21))-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg)
![Western Blot: TMED7 Antibody [NBP2-15141] - A: 293T B: A431 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize2/TMED7-Antibody-Western-Blot-NBP2-15141-img0002.jpg)
![Western Blot: Dynactin 6 Antibody [NBP1-80616] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/Dynactin 6 Antibody-Western Blot-NBP1-80616-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynactin 6 Antibody [NBP1-80616] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Dynactin-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80616-img0002.jpg)
![Western Blot: Dynactin 6 Antibody [NBP1-80616] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/Dynactin-6-Antibody-Western-Blot-NBP1-80616-img0005.jpg)
![Western Blot: SSSCA1 Antibody [NBP2-13387] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/SSSCA1 Antibody-Western Blot-NBP2-13387-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SSSCA1 Antibody [NBP2-13387] - Staining of human cell line U-251 MG shows positivity in cytoplasm.](http://images.novusbio.com/fullsize2/SSSCA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13387-img0003.jpg)
![Immunohistochemistry-Paraffin: SSSCA1 Antibody [NBP2-13387] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize2/SSSCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-13387-img0001.jpg)
![Immunohistochemistry: IFI27 Antibody [NBP1-84745] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize2/IFI27-Antibody-Immunohistochemistry-NBP1-84745-img0004.jpg)
![Western Blot: 26S proteasome subunit 9 Antibody [NBP2-47560] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line MCF-7](http://images.novusbio.com/fullsize2/26S-proteasome-subunit-9-Antibody-Western-Blot-NBP2-47560-img0002.jpg)
![Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP2-47560] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/26S-proteasome-subunit-9-Antibody-Immunohistochemistry-Paraffin-NBP2-47560-img0003.jpg)
![Immunofluorescence: 26S proteasome subunit 9 Antibody [NBP2-47560] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/26S-proteasome-subunit-9-Antibody-Immunofluorescence-NBP2-47560-img0004.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2 Antibody (2D9)-Western Blot-NBP2-00776-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/Cdk2 Antibody (2D9)-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0007.jpg)
![Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2 Antibody (2D9)-Immunohistochemistry-Paraffin-NBP2-00776-img0006.jpg)
![Western Blot: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCEAL1.](http://images.novusbio.com/fullsize2/TCEAL1 Antibody (2G10)-Western Blot-NBP2-46085-img0012.jpg)
![Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(](http://images.novusbio.com/fullsize2/TCEAL1-Antibody-(2G10)-Immunohistochemistry-NBP2-46085-img0011.jpg)
![Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize2/TCEAL1-Antibody-(2G10)-Immunohistochemistry-NBP2-46085-img0001.jpg)
![Western Blot: D4S234E Antibody [NBP1-87425] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/D4S234E Antibody-Western Blot-NBP1-87425-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP1-87425] - Staining of human cell line A-431 shows positivity in mitochondria.](http://images.novusbio.com/fullsize2/D4S234E-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87425-img0004.jpg)
![Immunohistochemistry-Paraffin: D4S234E Antibody [NBP1-87425] - Staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.](http://images.novusbio.com/fullsize2/D4S234E-Antibody-Immunohistochemistry-Paraffin-NBP1-87425-img0001.jpg)
![Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.](http://images.novusbio.com/fullsize2/p19ARF-Antibody-Western-Blot-NB200-106-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0004.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/CDK4-Antibody-Western-Blot-NBP1-31308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize2/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0010.jpg)
![Immunohistochemistry-Paraffin: Cdk4 Antibody [NBP1-31308] - Paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Cdk4 Antibody-Immunohistochemistry-Paraffin-NBP1-31308-img0006.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize2/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Western Blot: Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-AKT1 (NBP2-01725) and 2 ug/ml pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize2/Akt1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also.](http://images.novusbio.com/fullsize2/AKT1-[p-Ser473]-Antibody-(104A282)-Immunohistochemistry-Paraffin-NB100-56749-img0010.jpg)
![Western Blot: Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Analysis using Azide/BSA FREE version of NB100-56749. Analysis of phospho AKT using phospho AKT antibody at 2 ug/ml against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/Akt1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0007.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0004.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0006.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Immunohistochemistry-NB500-106-img0001.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.](http://images.novusbio.com/fullsize2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0005.jpg)
![Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.](http://images.novusbio.com/fullsize2/cleaved-Retinoblastoma-1-Antibody-(172C1094)-Western-Blot-NB100-56598-img0005.jpg)
![Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.](http://images.novusbio.com/fullsize2/cleaved-Retinoblastoma-1-Antibody-(172C1094)-Immunohistochemistry-NB100-56598-img0004.jpg)
