p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

Western blot analysis of A431 lysate using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free.

Western Blot Analysis of NIH/3T3 cell lysate using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free.

IHC analysis of formalin-fixed, paraffin-embedded human bladder. Strong nuclear staining using p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: KIP1/1355R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human CDKN1B protein (Uniprot: P46527)
• Localization: Cytoplasm, Endosome, Nucleus
• Specificity: This antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDKN1B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for p27/Kip1 Antibody (KIP1/1355R) - Azide and BSA Free

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1B