p27/Kip1 Antibody (341116)

p27/Kip1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Rat Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB22561) overnight at 4 °C. Tissue was stained with the Human p27 Immunohistochemistry Detection Kit (brown; Catalog # CTS2256) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: 341116
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

p27/Kip1 Antibody (341116) Summary

• Immunogen: E. coli-derived recombinant human p27/Kip1
  Met1-Thr198
  Accession # P46527
• Specificity: Detects human p27/Kip1 in direct ELISAs. Specificity was demonstrated by comparing staining on tissue sections of normal versus p27/Kip1 knockout mice.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CDKN1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p27/Kip1 Antibody (341116)

• CDKN1B
• CDKN4
• cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• cyclin-dependent kinase inhibitor 1B
• Cyclin-dependent kinase inhibitor p27
• Kip1
• KIP1P27KIP1
• MEN1B
• MEN4
• p27
• p27Kip1

Background

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Human p27/Kip1 shares 88% and 87% amino acid sequence identity with mouse and rat p27/Kip1, respectively, while mouse and rat p27/Kip1 share 96% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1B
• Uniprot:
  • P46527()