p23/PTGES3 Antibody (JU09-31)

Western Blot: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.Positive control:Lane 1: Mouse brain tissue Lane 2: SK-Br-3Lane 3: Rat lung tissue

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Staining p23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Analysis of paraffin-embedded rat testis tissue using anti-p23 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Staining p23 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Analysis of paraffin-embedded human tonsil tissue using anti-p23 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Analysis of paraffin-embedded human colon cancer tissue using anti-p23 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JU09-31) [NBP2-75603] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-p23 antibody. Counter stained with hematoxylin.

Product Discontinued

p23/PTGES3 Antibody (JU09-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human p23/PTGES3 aa 43-92 / 160. (SwissProt: Q15185 Human; SwissProt: Q9R0Q7 Mouse; SwissProt: P83868 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTGES3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p23/PTGES3 Antibody (JU09-31)

• CPGES
• cPGESp23
• Cytosolic prostaglandin E2 synthase
• Hsp90 co-chaperone
• p23
• P23cytosolic prostaglandin E synthase
• Progesterone receptor complex p23
• prostaglandin E synthase 3 (cytosolic)
• prostaglandin E synthase 3
• PTGES3
• TEBP
• TEBPEC 5.3.99.3
• Telomerase-binding protein p23
• unactive progesterone receptor, 23 kD

Background

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP 70 and HSP 90), immunosuppressant binding proteins called immunophilins (the FK 506 binding proteins, FKBP 52 & FKBP 54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP 70, HSP 90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP 90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGES3