p23/PTGES3 Antibody (JJ6)

Images

 
Western Blot: p23/PTGES3 Antibody (JJ6) [NB110-96879] - analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 . Load: 15 ug protein. Block: 1.5% BSA for 30 ...read more
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. ...read more
Immunohistochemistry: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC ...read more
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ6) [NB110-96879] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody at 1:100 for 12 hours at ...read more

Product Details

Summary
Reactivity Hu, Mu, Ch, Gp, Rb, YeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, MiAr
Clone
JJ6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

p23/PTGES3 Antibody (JJ6) Summary

Immunogen
Recombinant human full length p23 protein
Localization
Cytoplasm
Specificity
Detects approx 23kDa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTGES3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:2000
Application Notes
0.5 ug/ml of p23 Antibody was sufficient for detection of p23 in 20 ug of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Control
p23/PTGES3 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for p23/PTGES3 Antibody (JJ6)

  • CPGES
  • cPGESp23
  • Cytosolic prostaglandin E2 synthase
  • Hsp90 co-chaperone
  • p23
  • P23cytosolic prostaglandin E synthase
  • Progesterone receptor complex p23
  • prostaglandin E synthase 3 (cytosolic)
  • prostaglandin E synthase 3
  • PTGES3
  • TEBP
  • TEBPEC 5.3.99.3
  • Telomerase-binding protein p23
  • unactive progesterone receptor, 23 kD

Background

p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the Hsp90-progesterone receptor complex along with hsp70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTGES3
Entrez
Uniprot