p21/CIP1/CDKN1A Antibody (WA-1 (HJ21))

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Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
WA-1 (HJ21)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) Summary

Immunogen
Recombinant human p21 protein was used as immunogen to generate the antibody.
Localization
Nuclear
Specificity
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location: 6p21.31 Use in ELISA has been reported in literature.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Use in Western blot reported in scientific literature (PMID 28137862).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-29463 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for p21/CIP1/CDKN1A Antibody (WA-1 (HJ21))

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

p21 (WAF1), a member of the cycle dependent kinase (CDK) inhibitor family, was first identified as a tumor suppressor or anti-oncogenic protein and negative regulator of the cell cycle. Over the years it became apparent that p21 has broad functions in regulating fundamental cellular programs including proliferation, differentiation, migration, senescence, and has both anti-oncogenic and oncogenic properties (reviewed in Romanov, 2012). Many antibody and other studies have demonstrated that p21 levels are frequently elevated in situations associated with reduced proliferation, including differentiation and senescence, and decreased in proliferative states. Subcellular localization may significant and nuclear antibody staining may be indicative of p21 functioning as a cell cycle inhibitor, tumor suppressor, or in a senescence program. In contrast, cytoplasmic antibody staining may indicate p21 is acting an oncogene through regulatation of migration, proliferation, or apoptosis.

It is interesting to note that p21 expression is induced by wild type, but not mutant p53. The HJ21/WA-1 antibody clone has been used to demonstrate this phenomenon by western blot (Blaydes, 2001). The inability of mutant p53 to induce p21 essentially means that normal functions of p21 are compromised when p53 gene mutations are present. Since p53 gene mutations are present in up to 50% of human cancers, the loss of p21 function in cancer is significant.

In its normal functioning state, p21 binds to cyclin/CDK complexes. When it binds to these complexes, it inhibits their kinase activity which, in turn, stops cell cycle progression and hence p21 gains its reputation as a mitotic cell cycle inhibitor. In this regard, the antibody showed that decreased Cdk2-cyclin E1 activity corresponded with a decrease in cyclin E1 and increase in p21 protein levels (White, 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for p21/CIP1/CDKN1A Antibody (NBP2-29463)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-29463 Applications Species
Huang X, Huang R, Gou S et al. Platinum(IV) complexes conjugated with phenstatin analogue as inhibitors of microtubule polymerization and reverser of multidrug resistance Bioorg. Med. Chem. 2017 Jul 08 [PMID: 28728896] (Human) Human
Xiang J, Yang S, Xin N et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome Proc. Natl. Acad. Sci. U.S.A Jan 30 2017 [PMID: 28137862] (WB, Mouse) WB Mouse
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID: 26400209] (IHC, Mouse) IHC Mouse
Romanov VS, Pospelov VA, Pospelova TV. Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis. Biochemistry (Mosc). 2012 Jun [PMID: 22817456]
Koike M, Yutoku Y, Koike A. Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation. Biochem Biophys Res Commun. 2011 Aug 19 [PMID: 21787748]
Nobre RJ, Herraez-Hernandez E, Fei JW et al. E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures. J Virol. 2009 Apr [PMID: 19153227] (WB, IHC-P, Human)

Details:
Human keratinocyte oragnotypic raft cultures: IHC (paraffin), Fig3A; WB, Fig 3B.
WB, IHC-P Human
Rossner P Jr, Binkova B, Milcova A et al. Air pollution by carcinogenic PAHs and plasma levels of p53 and p21(WAF1) proteins. Mutat Res. 2007 Jul 1 [PMID: 17408702] (Human)

Details:
Direct ELISA (human blood plasma), CLONE SPECIFC Tables 1-3. Note: The specificity of the p21 antibody for p21 was validated with recombinant p21 by direct ELISA in Tables 1-3.
Human
Scott MT, Ingram A, Ball KL. PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein. EMBO J. 2002 Dec 16 [PMID: 12485998] (WB)

Details:
WB: (HeLa), Fig 3D.
WB
Rossner P Jr, Binkova B, Chvatalova I et al. Acrylonitrile exposure: the effect on p53 and p21(WAF1) protein levels in the blood plasma of occupationally exposed workers and in vitro in human diploid lung fibroblasts. Mutat Res. 2002 May 27 [PMID: 12034325] (WB)

Details:
Direct ELISA (human blood plasma), Tables 3-5; WB (primary human lung fibroblasts), Fig 3. The specificity of the p21 antibody for p21 was validated with recombinant p21 by direct ELISA in Tables 3-5.
WB
Blaydes JP, Luciani MG, Pospisilova S et al. Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription. J Biol Chem. 2001 Feb 16 [PMID: 11078726] (WB)

Details:
WB (SAOS), Fig 5.
WB
Show All 11 Publications.

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FAQs for p21/CIP1/CDKN1A Antibody (NBP2-29463). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know the concentration of NBP2-29463.
    • NBP2-29463 (p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) has the concentration of 0.2 mg/ml.

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Bioinformatics

Gene Symbol CDKN1A
Entrez
Uniprot