p21/CIP1/CDKN1A Antibody (SPM306)

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Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (SPM306) [NBP2-44526] - Human Colon Carcinoma stained with p21 Monoclonal Antibody (SPM306).

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, IHC-P, ICC/IF
Clone
SPM306
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

p21/CIP1/CDKN1A Antibody (SPM306) Summary

Immunogen
Human recombinant p21 protein
Localization
Nuclear
Specificity
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 6p21.31
Immunohistochemistry-Paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p21/CIP1/CDKN1A Lysate (NBP2-64699)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for p21/CIP1/CDKN1A Antibody (SPM306)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDKN1A
Entrez
Uniprot