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p21/CIP1/CDKN1A Antibody (1032)

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Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Genetic Strategies: Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was ...read more
Biological Strategies: Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of: A. 30 ug HCT116 whole cell lysate/extract (untreated) B. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin ...read more
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 ...read more
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Human lung cancer. p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - p21 Cip1 antibody [1032] detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 ...read more
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody ...read more
Biological Strategies: Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
Clone
1032
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

  

Genetic Strategies

    

Order Details

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p21/CIP1/CDKN1A Antibody (1032) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
Application Notes
FACS, IHC-P-Assay dependent
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for p21/CIP1/CDKN1A Antibody (1032)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by flow cytometry.
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Bioinformatics

Gene Symbol CDKN1A