p21/CIP1/CDKN1A Antibody (1032)

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Genetic Strategies: Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was ...read more
Biological Strategies: Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of: A. 30 ug HCT116 whole cell lysate/extract (untreated) B. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin ...read more
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 ...read more
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Human lung cancer. p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - p21 Cip1 antibody [1032] detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 ...read more
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody ...read more
Biological Strategies: Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
Clone
1032
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

p21/CIP1/CDKN1A Antibody (1032) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
Application Notes
FACS, IHC-P-Assay dependent
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for p21/CIP1/CDKN1A Antibody (1032)

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1A