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p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free

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Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Whole cell protein from mouse Neuro2A and MEF cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Staining of sections from a wild type mouse testis taken at P11 were probed with NB200-174 (green). Sections were stained with DAPI to reveal the ...read more
Flow (Intracellular): p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - An intracellular stain was performed on HEK293 cells with p14ARF (5-C3-1) antibody NB200-174 (blue) and a matched isotype control MAB0061 (orange) [A]. ...read more
Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Aberrantly upregulated p19ARF/CDKN2A antagonizes TFH differentiation and survival. p19ARF/CDKN2A and Bcl6 interaction capacity. WT or mutant p19ARF/CDKN2A was ...read more
Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Analysis of endogenous p19Arf. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo ...read more
Knockout Validated: p19ARF/CDKN2A Antibody (5-C3-1) [NB200-174] - Detection of p19Arf. Wild type mouse embryo fibroblasts (MEF) at passage 5 (panels 1, 2) and NIH3T3 cells, which have deleted the Arf gene (panels 3, 4) ...read more
Aberrantly upregulated p19Arf antagonizes TFH differentiation and survival. b) p19Arf and Bcl6 interaction capacity. WT or mutant p19Arf was co-transfected with WT Bcl6 expression vector into 293T cells. Cell lysates ...read more
Loss of P19Arf enhances tumor growth and primary lung fibroblasts induce p19Arf in response to leucine deprivation (LD). (B) Western blot for p19Arf protein expression in WT and Arf-null fibroblasts after LD for the ...read more
HPV status and EphrinB1 contribute to neurite outgrowth. a Quantified neurite outgrowth of PC12 cells following exosome stimulation with HPV negative (−) or positive (+) human cell lines; statistical analysis by ...read more

Product Details

Summary
Reactivity Hu, Mu, SyHa(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO
Clone
5-C3-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free Summary

Immunogen
A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]
Epitope
The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Cdkn2a
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 5 u/gml
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 27177758)
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Western Blot 1:500-1:1000
Application Notes
In Western blot a band can be seen at ~19 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB200-174 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for p19ARF/CDKN2A Antibody (5-C3-1) - BSA Free

  • alternative reading frame
  • ARF
  • ARF-INK4a
  • CDK4I
  • CDKN2A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • cyclin-dependent kinase inhibitor 2A, isoform 3
  • cyclin-dependent kinase inhibitor 2A, isoforms 1/2
  • cyclin-dependent kinase inhibitor protein
  • INK4
  • INK4a
  • Ink4a/Arf
  • INK4a-ARF
  • mitochondrial smARF'
  • MTS1
  • p14
  • p14ARF
  • P16
  • p16(INK4a)
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • p19<ARF>
  • p19ARF
  • Pctr1

Background

ARF (alternative reading frame; p14ARF in human, p19ARF in mouse) is a tumor suppressor that serves as a sensor of hyper-proliferative signals, resulting in p53-dependent growth arrest and apoptotic cell death. ARF interacts with several proteins including COMMD1, CDKN2AIP, TBRG1, E4F1, C1QBP etc. and can bind to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI as well as UBE2I/UBC9. ARF has the capability of inducing cell cycle arrest in G1 as well as G2 phases. While ARF is not expressed at significant levels in most normal tissues, oncogene activation triggers ARF overexpression, resulting in inhibition of MDM2 ubiquitin ligase and stabilization of p53. It is induced by progesterone as well as Ras/DMTF1, and is downregulated through NFkB p65 subunit (RELA). Inhibition of the p53 pathway, most commonly via mutations in p53 itself, inactivation of ARF, or amplification of MDM2 has been proposed to be a crucial step in pathogenesis of most human malignancies. Moreover, mice with null ARF have been shown to develop tumors rapidly and to display delayed mammary gland involution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p19ARF/CDKN2A Antibody (NB200-174)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(3)
ICC/IF
(4)
IF/IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(8)
All Applications
Filter By Species
Human
(3)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB200-174 Applications Species
Weidenhammer L, Liu H, Luo L et al. Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination STAR Protocols 2023-03-01 [PMID: 36853662]
Neupane R BMI1 is a context-dependent tumor suppressor that is a barrier to dedifferentiation in non-small cell lung adenocarcinoma Thesis 2019-05-23 (IF/IHC, Mouse) IF/IHC Mouse
Maru Y, Tanaka N, Tatsumi Y et al. Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition Oncogenesis 2021-06-25 [PMID: 34172714] (WB, Mouse) WB Mouse
Suzuki T, Katada E, Mizuoka Y, et al. A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing Nucleic acids research 2021-01-27 [PMID: 33503245] (WB, Mouse) WB Mouse
Deland, K, Starr, B F Et al. Tumor genotype dictates radiosensitization after Atm deletion in primary brainstem glioma models. J Clin Invest 2021-01-04 [PMID: 32990677] (WB, Mouse) WB Mouse
Santelli A, Sun I, Eirin A, et al. Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles J Am Heart Assoc 2019-08-22 [PMID: 31433703] (FLOW, Human) FLOW Human
Li F, Zeng Z, Xing S et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf Nat Commun 2018-12-21 [PMID: 30575739] (WB, Human) WB Human
Baek KH, Ryeom S. Detection of Oncogene-Induced Senescence In Vivo. Methods Mol. Biol. 2016-11-04 [PMID: 27812880]
Moding EJ, Min HD, Castle KD et al. An extra copy of p53 suppresses development of spontaneous Kras-driven but not radiation-induced cancer JCI Insight 2016-07-07 [PMID: 27453951]
Li D, Fu J, Du M et al. Hepatocellular carcinoma repression by TNFalpha-mediated synergistic lethal effect of mitosis defect-induced senescence and cell death sensitization. Hepatology. 2016-05-14 [PMID: 27177758] (IHC-P, ICC/IF, Human, Mouse) IHC-P, ICC/IF Human, Mouse
Show All 23 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p19ARF/CDKN2A Antibody (NB200-174). (Showing 1 - 1 of 1 FAQs).

  1. We would like to confirm if the immunogen sequence is a synthetic peptide containing amino acids 54-75 (RRGPHRNPGPGDDDGQRSRSSS) of the murine p19ARF. And also, the epitope recognized is between amino acids 62-75 of murine p19ARF.
    • I can confirm that the immunogen sequence for our product NB200-174 is aa 54-75 of the mouse Cdkn2a (also known as p19ARF) protein (Uniprot Site). The epitope recognized by this protein is aa 62-75.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol Cdkn2a