p19ARF Antibody (5-C3-1) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, SyHa(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
5-C3-1
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 488

p19ARF Antibody (5-C3-1) [DyLight 488] Summary

Immunogen
A synthetic peptide containing amino acids 54-75 of the murine p19ARF. [UniProt# Q64364]
Epitope
The epitope recognized is between amino acids 62-75 of murine p19ARF.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CDKN2A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This p19ARF (5-C3-1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections, Immunoprecipitation, Flow Cytometry and Western blot, where a band can be seen at approximately 19 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse. Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p19ARF Antibody (5-C3-1) [DyLight 488]

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p19ARF Antibody (NB200-174G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB200-174APC
Biotin Labeled NB200-174B
DyLight 350 Labeled NB200-174UV
DyLight 405 Labeled NB200-174V
DyLight 550 Labeled NB200-174R
DyLight 650 Labeled NB200-174C
DyLight 680 Labeled NB200-174FR
DyLight 755 Labeled NB200-174IR
FITC Labeled NB200-174F
HRP Labeled NB200-174H
PE Labeled NB200-174PE
PerCP Labeled NB200-174PCP

Additional p19ARF Products

p19ARF NB200-174G

Bioinformatics Tool for p19ARF Antibody (NB200-174G)

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Research Areas for p19ARF Antibody (NB200-174G)

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Blogs on p19ARF.

You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.

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Bioinformatics

Gene Symbol CDKN2A