p19ARF/CDKN2A Antibody (5-C3-1) [Allophycocyanin]

Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [Allophycocyanin] - Conducted the antibody trial with two different dilutions of p19ARF/CDKN2A Antibody (5-C3-1) [PE] (Catalog # NB200-174PE) (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. Image from a verified customer review.

• Reactivity: Hu, Mu, SyHa(-)
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO
• Clone: 5-C3-1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

p19ARF/CDKN2A Antibody (5-C3-1) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]
• Epitope: The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Cdkn2a
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB200-174. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for p19ARF/CDKN2A Antibody (5-C3-1) [Allophycocyanin]

• alternative reading frame
• ARF
• ARF-INK4a
• CDK4I
• CDKN2A
• Cyclin-dependent kinase 4 inhibitor A
• cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
• cyclin-dependent kinase inhibitor 2A
• cyclin-dependent kinase inhibitor 2A, isoform 3
• cyclin-dependent kinase inhibitor 2A, isoforms 1/2
• cyclin-dependent kinase inhibitor protein
• INK4
• INK4a
• Ink4a/Arf
• INK4a-ARF
• mitochondrial smARF'
• MTS1
• p14
• p14ARF
• P16
• p16(INK4a)
• p16-INK4
• P16INK4A
• p16-INK4a
• P19
• p19<ARF>
• p19ARF
• Pctr1

Background

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Cdkn2a